混料试验设计在纤维素酶复配中的应用研究  

Mixture Design Optimization of Cellulase Complex Formulation

作　　者：林增祥[1] 张红漫[2] 严立石[1] 陈敬文[1] 胡龄[1] 于文涛[1] 黄和[1] 

机构地区：[1]南京工业大学制药与生命科学学院,材料化学工程国家重点实验室,江苏南京210009 [2]南京工业大学应用化学系,江苏南京210009

出　　处：《食品科学》2009年第15期169-171,共3页Food Science

基　　金：江苏省自然科学基金项目(BK2005114);国家自然科学基金项目(20576054);国家"863"计划项目(2006AA02Z240)

摘　　要：通过对三个不同来源的纤维素酶进行复配以提高纤维素酶的水解效率,以玉米秸秆酶水解液中还原糖浓度为评价指标,采用混料试验设计优选纤维素酶的复配条件并建立数学拟合方程。结果表明:最佳的酶复配条件为泽生纤维素酶与杰能科纤维素酶体积比1:1时纤维素水解效率最高,比使用单一纤维素酶效率提高了39.5%。利用混料试验设计可以对纤维素酶复配条件进行优化。Three different commercial cellulase preparations were used to prepare complex enzyme to increase the hydrolysis efficiency of cellulase. The formulation of cellulose complex was optimized by mixture design to attain the maximum concentration of reduced sugar in hydrolysate solution and a mathematical model describing the relationship of concentration of reduced sugar with volumes of mixed cellulases was developed. A mixture of Zesheng cellulase and Genencor cellulase at a volume ratio of 1： 1 resulted in the maximum hydrolysis efficiency higher than that of individual cellulase by 39.5%. Mixture design is available for the optimization of cellulase complex formulation.

关 键 词：混料设计 纤维素酶 复配 纤维素 

分 类 号：Q396[生物学—遗传学]