紫外诱变赤灵芝原生质体选育高产有机锗菌株的研究  

UV Mutagenization of Protoplast for Screening and Breeding of High-yield Organogemanium-producing Strains of Ganoderma lucidum

作　　者：董玉玮[1] 苗敬芝[1] 曹泽虹[1] 高明侠[1] 吕兆启[1] 

机构地区：[1]徐州工程学院食品工程学院,江苏徐州221008

出　　处：《食品科学》2009年第15期188-192,共5页Food Science

基　　金：江苏省教育厅课题(08KJD18002);徐州工程学院校级科研项目(XKY2008228)

摘　　要：对赤灵芝原生质体制备、再生及紫外诱变育种、筛选有机锗高产菌株进行研究。结果表明:赤灵芝原生质体制备与再生的最佳条件为菌龄96h,复合酶液浓度为2%溶壁酶、1%蜗牛酶和1%纤维素酶,温度31℃,时间2h,获得赤灵芝原生质体产量为4.33×106个/ml,再生率达0.00275%。原生质体最佳紫外诱变条件为:15W紫外灯,距离30cm,照射30s,致死率为66.7%。最终选育出3株高产有机锗诱变株,有机锗含量分别比出发菌株提高了113.58%、105.87%、103.86%。Preparation, regeneration, UV mutagenization of Ganoderma lucidum protoplast were studied to obtain high-yield strains producing organogemanium. Under optimized conditions for the preparation and regeneration of Ganoderma lucidum protoplast including cell age 96 h, lywallzyme concentration 2%, glusulase concentration 1%, cellulase concentration 1%, and temperature 31 ~Cfor an enzymolysis duration of 2 h, the protoplast yield and regeneration rate were found to be 4.33 × 10^6 protoplasts/ml and 0.00275%, respectively. When Ganoderma lucidum protoplast was irradiated for 30 s with 15 W ultraviolet lamp 30 cm away from it, the lethality was 66.7% and three high-yield strains were obtained after two passages, the organogemanium contents in whose dried mycelia were higher than that of starting strain by 113.58%, 105.87% and 103.86% separately.

关 键 词：紫外诱变 原生质体 赤灵芝 有机锗 

分 类 号：S567.31[农业科学—中草药栽培]