胃肠道伤害性刺激诱导大鼠孤束核、视上核、室旁核内FOS蛋白的表达  

GASTROINTESTINAL TRACT NOXIOUS STIMULATION-INDUCED FOS EXPRESSION IN NUCLEUS OF SOLITARY TRACT, SUPRAOPTIC NUCLEUS AND PARAVENTRICULAR NUCLEUS IN RAT

作　　者：李光昭[1] 韩东日[1] 初春平[1] 崔尚允[1] 

机构地区：[1]延边大学医学院局部解剖学教研室,延吉市133000

出　　处：《解剖学杂志》1998年第4期317-320,共4页Chinese Journal of Anatomy

摘　　要：用抗FOS免疫组化技术,观察胃肠道伤害性刺激诱导大鼠孤束核、视上核、室旁核内c-fos的表达,并结合抗TH免疫双重染色技术,探讨孤束核内儿茶酚胺能神经元与FOS蛋白的关系.结果表明:FOS阳性细胞主要分布于孤束核的连合亚核、内侧亚核以及背侧周边区,说明孤束核是内脏伤害性信息初级传入冲动的直接反应区.在下丘脑内主要分布于视上核和室旁核,提示视上核和室旁核在内脏伤害性刺激的传递中起中继作用.在双标切片中,FOS/TH双标细胞集中分布于孤束核的内侧亚核和背侧周边区,说明孤束核内儿茶酚胺能神经元也参与内脏伤害性刺激反应.Gastrointestinal tract noxious stimulation-induced Fos expression in the nucleus of solitary tract, supraoptic nucleuse and paraventricular nucleus in rat was observed by Fos immunohis-tochemical method and the relationship between catecholaminergic neurons and Fos protein in the nucleus of the solitary tract was investigated by combing Fos immunohistochemical method with tyrosine hydroxylase immunohistochemical staining. The result showed that the Fos-like im-munoreactive (Fos-LI) neurons were mainly distributed in the commissural subnucleus, medial subnucleus and peridorsal region of the nucleus of the solitary tract, indicating that the nucleus of solitary tract was the direct reactive region of primary afferent impulse of the visceral noxious stimulation information. The Fos-LI neurons were mainly distributed in the supraoptic nucleus and paraventricular nucleus of the hypothalamus, indicating that the two nuclei had relaying action in the transmission of visceral noxious stimulation. In double-labelling sections, Fos/TH double labelled neurons were mainly localized in the commissural subnucleus, medial subnucleus and peridorsal region of the nucleus of solitary tract, indicating that catecholaminergic neurons in the nucleus of solitary tract were involved in the visceral noxious stimulation reaction.

关 键 词：胃肠道 孤束核 下丘脑 大鼠 刺激 视上核 室旁核 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学] R338.3[医药卫生—基础医学]