菱角粗多糖对肿瘤细胞抑制作用  

Antitumor effect of crude polysaccharides components from water-caltrop

作　　者：牛凤兰[1] 董卿[1] 巩宏伟[1] 哈卿[1] 孙文怡[2] 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]吉林师范大学

出　　处：《中国公共卫生》2009年第8期1005-1006,共2页Chinese Journal of Public Health

基　　金：吉林省科技厅重点资助项目(20070424);长春市科技局重点项目(2006219);吉林省高新技术产业重点支持项目(20051564)

摘　　要：目的分离提取菱角粗多糖并进行体外抑瘤作用研究。方法采用乙醇分级沉淀法分离菱角粗多糖,苯酚硫酸法测定其糖含量,并采用四甲基偶氮噻唑蓝(MTT)法测定其对肿瘤细胞增殖的抑制作用,流式细胞术分析其中多糖组分F3诱导人脑胶质瘤细胞U251凋亡作用。结果从菱角中分离得到3个粗多糖组分,菱角粗多糖具有抑制肿瘤细胞增殖及诱导肿瘤细胞凋亡的作用,流式细胞仪检测图谱上出现明显的亚二倍体峰,3个组分浓度在7.81,15.63,31.25,62.50,125.00μg/mL范围内对Hela和U251细胞增殖有抑制作用,最高抑制率均达70%以上,并存在明显的剂量效应关系。结论菱角粗多糖能够抑制Hela和U251细胞肿瘤的增殖,并诱导其凋亡。Objective To extract the crude polysaccharides from water-caltrop and to study their antitumor effect in vitro. Methods Ethanol fractionation method was used to separate crude polysaccharides from water-caltrop. MTT method was used to evaluate the antitumor effect of crude polysaccharides in vitro. Flow cytometry method was used to detect the apoptosis of U251 cells induced by the crude polysaccharide. Results Three kinds of polysaccharide compounds were obtained from the water-caltrop. The results of MTT assay showed that different concentration（7. 81,15.63,31.25,62. 50 and 125.00 μg/mL）of the crude polysaccharides could significantly inhibit the proliferation of Hela and U251 cells in a dosedependent manner and induce the cell apoptosis. The number of two kind ceils gradually decreased with the increasing of concentration of the crude polysaccharides. Conclusion The results proved that crude polysaccharide could inhibit the Hela and U251 cells proliferation and induce U251 cell apoptosis.

关 键 词：菱角 粗多糖 细胞凋亡 

分 类 号：R730.26[医药卫生—肿瘤]