基于PCR法快速检测扩展青霉  

Rapid Detection of Penicillium Expansum Based on PCR Technology

作　　者：袁晖[1] 樊明涛[1] 刘玉波[1] 杨永恒[1] 李瑜[1] 焦凌霞[2] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]河南科技学院食品学院,河南新乡453003

出　　处：《西北农业学报》2009年第4期315-318,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：教育部博士点基金(200807120017);陕西省科技攻关基金(2007K01-12)

摘　　要：对PCR快速检测扩展青霉的方法进行了研究,重点考察了退火温度对检测结果的影响,评价了该方法的灵敏度,并考察了该法在苹果样品检测中的应用效果。结果表明,退火温度为51~61℃,扩展青霉3.5425 DNA扩增效果良好,纯菌培养所得菌体的DNA质量浓度的检测限为2.9×10^-3μg/mL,菌液的检测限低于15 cfu/mL,苹果样品对菌体DNA检测结果无影响。PCR was used to study Penicillium expansum in this study,The annealing temperature and sensitivity was measured including DNA from samples of inoculated apples. The result showed that the amplification was good between 51-61℃, 2.9× 10^-3 ug/mL DNA in pure culture can be detected. The detection limit was lower than 15cfu/mL in liquid culture. There was no interference from apple DNA.

关 键 词：苹果 扩展青霉 PCR 检测方法 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]