液相微萃取-高效液相色谱法分析葡萄汁中多酚类化合物  

Determination of polyphenols in grape juice by liquid-phase microextraction coupled with high performance liquid chromatography

作　　者：胡玉玲[1] 常蓓蓓[1] 罗学军[1] 李攻科[1] 

机构地区：[1]中山大学化学与化学工程学院,广州510275

出　　处：《分析试验室》2009年第8期22-25,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20775095;20705042);广东省自然科学基金(06023094)项目资助

摘　　要：建立了一种基于液相微萃取与高效液相色谱联用技术测定葡萄汁中鞣花酸、白藜芦醇和槲皮素的分析方法。比较了单液滴液相微萃取和中空纤维液相微萃取两种萃取模式，选择了单液滴液相微萃取作为3种多酚类化合物的液相微萃取模式。考察了搅拌速度、萃取时间、料液相pH和料液相离子强度的影响。鞣花酸、白藜芦醇和槲皮素的富集倍数分别为48．4、79．4和155．8，方法的线性范围为0．0050～5．0μg/mL，鞣花酸、白藜芦醇和槲皮素的检出限分别为0．015，0．0020，0．0080μg／mL，相对标准偏差分别为2．0％，1．8％和1．7％。用于实际样品葡萄汁的分析，加标回收率在81．9％～102．3％之间。A novel method for the determination of ellagic acid, resveratrol and quercetin in grape juice by liquidphase microextraction （LPME） coupled with high performance liquid chromatography （HPLC） was developed. The resuhs of two different modes of LPME were eompared, and the single drop microextraction exhibited better extraction efficiency. Effects of stirring speed, extraction time, ionic strength, and pH were investigated. The enrichment factors of ellagic acid, resveratrol and quercetin were 48.4, 79.4 and 155.8, respectively. The linear range of the method was from 0.0050 to 5.0 μg/mL. The detection limits of ellagic acid, resveratrol and quercetin were 0.015, 0.0020, 0.0080μg/mL, and the RSDs were 2.0%, 1.8% and 1.7%, respectively. The recoveries were from 81.9% to 102.3% for grape juice samples. The method with little solvent consumption was simple, fast, sensitive and suitable for the determination of polyphenols in plant samples.

关 键 词：液相微萃取 高效液相色谱 葡萄汁 多酚 

分 类 号：O557.7[理学—热学与物质分子运动论]