Real-time monitoring of DNAzyme cleavage process using fluorescent assay  

作　　者：Xiang Xian Meng Xiao Hai Yang Ke Min Wang Wei Hong Tan Qiu Ping Guo 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics, Engineering Center for Biomedicine,Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province,College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

出　　处：《Chinese Chemical Letters》2009年第8期990-994,共5页中国化学快报（英文版）

基　　金：supported in part by the National Key Basic Research Program of China(No.2002CB513110);Natural Science Foundation of China(No.90606003,No.20505007);Major International(Regional)Joint Research Program of Natural Science Foundation of China(No.20620120107);project supported by Hunan Provincial Natural Science Foundation of China(No.08JJ1002).

摘　　要：Detection of deoxyribozyme （DNAzyme） cleavage process usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe. The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time. Compared with traditional approach, this non-isotope method not only brought a convenient means to monitor the DNAzyme cleavage reaction, but also offered abundant dynamic data for choosing potential gene therapeutic agents. It provides a new tool for DNAzyme research, as well as a new insight into research on human disease diagnosis. Based on this method, 8- 17deoxyribozyme （8-17DNAzyme） against hepatitis C virus RNA （HCV-RNA） was designed and the cleavage process was studied in real time. ？2009 Ke Min Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.Detection of deoxyribozyme （DNAzyme） cleavage process usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe. The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time. Compared with traditional approach, this non-isotope method not only brought a convenient means to monitor the DNAzyme cleavage reaction, but also offered abundant dynamic data for choosing potential gene therapeutic agents. It provides a new tool for DNAzyme research, as well as a new insight into research on human disease diagnosis. Based on this method, 8- 17deoxyribozyme （8-17DNAzyme） against hepatitis C virus RNA （HCV-RNA） was designed and the cleavage process was studied in real time. ？2009 Ke Min Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.

关 键 词：DNAZYME CLEAVAGE Real time HCV-RNA 

分 类 号：Q522[生物学—生物化学] V263[航空宇航科学与技术—航空宇航制造工程]