用清除有机自由基DPPH法评价竹叶挥发油抗氧化能力  

Detection of antioxidative capacity of essential oils from the bamboo leaves by scavenging organic free radical DPPH

作　　者：何跃君[1] 岳永德[1] 汤锋[1] 

机构地区：[1]国际竹藤网络中心,北京100102

出　　处：《安徽农业大学学报》2009年第3期408-412,共5页Journal of Anhui Agricultural University

基　　金："十一五"国家科技支撑计划项目子课题(2006BAD19B08);国际竹藤网络中心基本科研业务费专项资金(06107-C27)共同资助

摘　　要：采用水蒸气蒸馏法从15种竹叶中提取挥发油,以TBHQ为对照,用DPPH.法研究竹叶挥发油对自由基的清除作用。结果表明,绿竹竹叶挥发油的得率最高为0.637%,而福建茶竿竹竹叶挥发油的得率仅为0.252%,水蒸气蒸馏法适宜于提取竹叶挥发油。15种竹叶挥发油均有一定的抗氧化活性,竹叶挥发油的抗氧化活性与挥发油的浓度呈正相关性,其中银丝竹竹叶挥发油的抗氧化活性最强,IC50值为3.605mg.mL-1,绿竹竹叶挥发油的IC50值为4.464mg.mL-1,短穗竹竹叶挥发油抗氧化活性最低,其IC50值为12.128mg.mL-1。综合研究结果表明,竹叶挥发油具有较高的应用价值,可作为天然抗氧化剂进一步开发和利用。The antioxidant capacities of essential oils obtained by steam distillation from the leaves of the 15 bamboo species, were evaluated using the DPPH assays. The yield of oils from the leaves of the 15 bamboo species was variable with the greatest amount obtained from Dendrocalamopsis oldhami （0. 637% ）, and the least from Pseudosasa amabilis （0. 252% ）. In the DPPH assays, the antioxidant capacity was evident in all the oils, but the antioxidant capacity of essential oil that obtained from Bambusa multiplex （IC50 = 3. 605 mg·mL^- 1 ） was greater than that from Brachystachym densiflorum （IC50 = 12. 128 mg · mL^-1 ）. The IC50 value of Dendrocalamopsis oldhami was 4. 464 mg·mL^-1. Antioxidant capacity was positively correlated with the concentration of essential oils. The data indicated that essential oils obtained from various bamboo leaves may be used as natural antioxidant.

关 键 词：竹叶 挥发油 1 1-二苯基苦基苯肼 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学] S795[轻工技术与工程—食品科学与工程]