高产刺糖菌素发酵菌株的选育  

Breeding of Production Strains of Spinosads

作　　者：朱明军[1] 江丹[1] 胡飞[2] 杜顺堂[3] 

机构地区：[1]华南理工大学生物科学与工程学院,广州510006 [2]华南理工大学轻工与食品学院,广州510006 [3]华粤行有限公司生物制药部,广州511442

出　　处：《农药》2009年第8期570-572,575,共4页Agrochemicals

基　　金：广东省科技计划资助项目(2007A020100001-12)

摘　　要：采用紫外线结合氯化锂以及硫酸二乙酯结合氯化锂2种常规诱变方法对刺糖多孢菌进行复合诱变,分别筛选鼠李糖抗性突变株、2-脱氧-D-葡萄糖抗性突变株和刺糖菌素抗性突变株,并结合自然分离纯化,最终获得高产菌株D-S-23,其产量达到89.57mg/L,较出发菌株提高了104.3%。The two routine methods of combined mutagenesis treatment, UV binding lithium chloride and diethyl sulfate binding lithium chloride, were used to mutagenize Saccharopolyspora spinosa. Some mutants resistant to rhanmose, 2-deoxy-D-glucose and spinosad were screened respectively. Combining natural isolation and purification, mutant strain D-S-23 was selected. Compared with the original strain, the titers of selected strain reached 89.57 mg/L, and the yield was increased by 104.3%.

关 键 词：刺糖菌素 选育 菌株 

分 类 号：TQ450.1[化学工程—农药化工]