晚期混合裂变产物内照射诱发体细胞 HPRT 基因位点突变的剂量效应关系研究  

STUDY ON THE RELATIONSHIP BETWEEN ACCUMULATIVE DOSE AND SOMATIC CELL MUTATION AT HPRT LOCUS INDUCED BY LATE FISSION PRODUCTS

作　　者：徐永忠[1] 赵经涌[1] 劳勤华[1] 

机构地区：[1]苏州医学院放射医学系

出　　处：《辐射研究与辐射工艺学报》1998年第3期165-168,共4页Journal of Radiation Research and Radiation Processing

摘　　要：运用多核细胞法及胞质分裂阻断微核（ＣＢＭＮ）法对５组Ｗｉｓｔａｒ雄性大鼠外周血淋巴细胞ＨＰＲＴ基因位点突变频率及微核进行检测。实验组静脉注射放射性比活度为３．６４×１０５Ｂｑ／ｍｌ的晚期混合裂变产物后１至９ｄ心脏穿刺取血。结果表明：静注后第１ｄ，累积剂量达１．７３ｃＳｖ时，与对照组相比，ＨＰＲＴ位点突变频率、、微核率均已显著增加（ｐ＜０．０１）；累积剂量与ＨＰＲＴ位点突变频率、微核细胞率、微核率间均可拟合成剂量效应函数Ｙ＝ａ＋ｂｌｎＸ；ＨＰＲＴ基因突变检测可望成为生物剂量计。With the technique of multinucleus cell and cytokinesis block micronucleus(CBMN) method, the relationships between mutations at HPRT loci, micronuclei and accumulative doses were analyzed. The results were as follows: On the first day after intravenous injection (accumulative dose 1.73cSv), by comparison with the control group, the HPRT mutant frequencies, micronucleus cell frequencies and micronucleus frequencies increased significantly (p<0.01). There existed a hemilogarithmic linear relationship between mutant frequencies at HPRT loci,micronucleus cell frequencies, micronucleus frequencies and accumulative doses, which could be described by following regression equation: Y= a+b lnX. A good linear relationship between HPRT mutant frequencies and micronucleus frequencies was observed. It is expected that the detection of HPRT locus mutation may soon be used as a biological dosimeter.

关 键 词：晚期 混合裂变产物 HPRT 基因突变 辐射生物学 

分 类 号：Q691[生物学—生物物理学] R818.03[医药卫生—放射医学]