脾虚型IBS模型WHBE兔的抗氧化活性研究  

Study on Anti-oxidation Activity in WHBE Rabbit with Irritable Bowel Syndrome of Spleen Deficiency Type

作　　者：张利棕[1] 徐剑钦[1] 潘永明[1] 王德军[1] 陈亮[1] 彭定国[1] 应华忠[1] 陈民利[1] 

机构地区：[1]浙江中医药大学动物实验研究中心／比较医学研究中心,杭州310053

出　　处：《实验动物与比较医学》2009年第4期273-276,共4页Laboratory Animal and Comparative Medicine

基　　金：浙江省公共科技基础平台项目（Dwpt-bz-12）;浙江省卫生高层次创新人才培养工程项目

摘　　要：目的观察脾虚型肠易激综合征（IBS）模型wHBE兔的抗氧化活性的变化。方法取WHBE兔和日本大耳白兔各12只，分别分成正常对照组6只、模型组6只，模型组采用湿热应激加灌服番泻叶煎剂的方法造成脾虚证肠易激综合征模型，造模结束后正常饲养10d进行自然恢复，观察实验兔血清中的SOD、MDA、GSH-Px、NO和NOS活性水平的变化。结果与正常对照组比较，模型组造模后WHBE兔和日本大耳白兔的血清SOD、GSH—Px活性水平显著降低（P〈0．05，P〈0．01）；而血清MDA含量、NO和NOS活性水平均显著性升高（P〈0．05，P〈0．01）；自然恢复10d后，WHBE兔血清GSH—Px活性水平仍显著降低（P〈0．05），MDA含量和NOS活性水平仍显著高于正常对照组（P〈0．05）；日本大耳白兔除NOS活性水平仍显著高于正常对照组（P〈0．05）外，其它各项抗氧化指标均无显著差异（P〉0．05）。结论脾虚型肠易激综合征模型WHBE兔的抗氧化活性明显降低，且恢复较为缓陵，其症状与人的临床表现近似，因此WHBE兔可能适合用于人脾虚型肠易激综合征的研究。Objective To observe the change of anti-oxidation activity in WHBE rabbit with irritable bowel syndrome of spleen deficiency type. Methods Twelve WHBE （white hair and black ear） rabbits and 12 Japanese white rabbits were randomly divided into two groups respectively, with 6 rabbits in normal control group and 6 rabbits in model group. The irritable bowel syndrome of spleen deficiency type rabbits model was made by using the methods of moist heat, stress and stimulation adding with taking senna decoction, and each model group rabbits were recovered naturally for 10 days after the successful establishment. The levels of superoxide dismutase （SOD）, the content of malondialdehyde （MDA）, the activities of glutathione peroxidase （GSH-Px）, nitric oxide （NO） and nitric oxide synthase （NOS） were determined. Results Compared with normal control groups, the activities of the serum SOD and GSH-Px were significantly decreased in WHBE rabbits and Japanese white rabbits after the successful establishment of the model （P〈0.05, P〈0.01）, the content of MDA, the activities of NO and NOS were significantly increased （P〈0.05, P〈0.01）. After 10 days nature recover, the activity of GSH- Px in the natural recovery groups was still lower, while the content of MDA and the activity of NOS in the WHBE rabbits were all significantly higher than those in the normal rabbits （P〈0.05）. All the antioxidation indexes in Japanese white rabbits returned to the normal level, except for the GSH-Px activity was still higher than normal control group （P〈0.05）. Conclusion The anti-oxidation activity of WHBE rabbit of irritable bowel syndrome of spleen deficiency type were significantly decreased similar to clinic in human, and recovered slowly, so the WHBE rabbits may be suitable for the study of irritable bowel syndrome of spleen deficiency type in huaman.

关 键 词：WHBE兔 日本大耳白兔 脾虚型肠易激综合征 抗氧化活性 

分 类 号：R-332[医药卫生] R574.4