二肽基肽酶Ⅳ在Ⅱ型糖尿病大鼠牙龈表达的影响  

Over expression of dipeptidyl peptidase Ⅳ/CD26 on gingiva of type 2 diabetic rats

作　　者：毛钊[1] 杨俭[2] 董震[1] 徐辉[1] 

机构地区：[1]江苏南京南京军区南京总医院口腔科,210002210029 [2]江苏南京南京医科大学基础医学院药理系,210029

出　　处：《牙体牙髓牙周病学杂志》2009年第8期431-434,445,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金面上项目资助(30772616);中国人民解放军南京军区十五医药卫生基金资助项目(02MB003)

摘　　要：目的:探讨高脂饮食和Ⅱ型糖尿病对大鼠牙龈组织中二肽基肽酶Ⅳ(DPP-Ⅳ/CD26)表达的影响。方法:将实验动物分为高脂饮食组(HF)、Ⅱ型糖尿病组(DM)和对照组(正常饮食),根据葡萄糖耐量试验异常确定Ⅱ型糖尿病动物模型成功。采用RT-PCR半定量和Western blot测定DPP-Ⅳ/CD26在大鼠肠(回肠)、肝脏、肾脏和牙龈组织中的表达情况。结果:与对照组比较,RT-PCR半定量测定HF组大鼠小肠、肾脏及牙龈组织内DPP-IVmRNA表达明显增加(151.58%,239.73%,169.7%,P<0.05),肝脏的DPP-IV无明显变化;DM组大鼠小肠、肾脏、肝脏和牙龈组织DPP-IV mRNA表达均明显增加(242.36%,324.93%,190.57%,267.4%,P<0.01)。Western blot证实HF组大鼠DPP-IV蛋白表达在小肠、肾脏、牙龈组织内中度增加(152.37%,154.17%,157.8%,P<0.05),而在肝脏无明显增加;DM组大鼠DPP-IV蛋白表达在小肠、肾脏、肝脏和牙龈组织均明显增加(222.5%,210.82%,225.43%,254.7%,P<0.01)。结论:在高脂饮食和Ⅱ型糖尿病时牙龈组织中DPP-IV表达上调,而这种上调可能是Ⅱ型糖尿病影响牙周炎发生发展的机制之一。AIM： To explore the expression of DPP - Ⅳ in intestine, kidney, liver and gingiva of rat in high fat diet and Type Ⅱ diabetes mellitus . METHODS ： Rats were devided into control, high fat diet （ HF ） and HF plus streptozotocin for type Ⅱ diabetes mellitus （DM） groups. Type Ⅱ diabetes mellitus rats were monitored with plasma glucose and insulin for 12 weeks and confirmed by the glucose/insulin tolerance test. The expressions of DPP - Ⅳ/ CD26 in the intestine, liver, kidney and gingiva in three groups were evaluated in mRNA and protein levels with RT - PCR and Western blotting. RESULTS： Compared to rats on the normal diet , the rats with a high fat diet significantly increased the expressions of DPP -Ⅳ（ mRNA / protein） 151.58% / 152.37% in the intestine; 239.73%/154.17% in the kidney and 169.7%/157.8% in the gingiva （ P 〈 0.05 ） , but there was no chang in the liver. The rats treated with high fat diet plus streptozotocin showed an increase in the expressions of DPP- Ⅳ（mRNA/protein） 242.36%/ 222.5% in intestine; 324.93% / 210.82% in kidney; 190.57% / 225.43% in liver and 267.4%/254.7% in gingiva （ P 〈 0.05 ）. CONCLUSION ： This study reports that an increase in the expressions of DPP - Ⅳ in gingiva is associated with high fat diet and Type Ⅱ diabetes mellitus . The evidence in this study suggests a hypothesis that the upregulation of the expressions of DPP - Ⅳ would contribute to a role in the development of gingivitis.

关 键 词：DPP-Ⅳ/CD26 Ⅱ型糖尿病 高脂饮食 牙龈组织 

分 类 号：R780.2[医药卫生—口腔医学]