响应面法优化超声辅助提取马钱子中生物碱的工艺研究  

Ultrasonic-assisted Extraction of Alkaloids from Semen Strychni by Response Surface Analysis

作　　者：邹建国[1] 徐小龙[1] 刘燕燕[1] 彭海龙[1] 徐彪[1] 罗平[1] 肖鉴谋[1] 

机构地区：[1]南昌大学环境与化学工程学院,江西南昌330031

出　　处：《食品科学》2009年第16期111-114,共4页Food Science

基　　金：江西省自然科学基金项目(2007GZH1924)

摘　　要：采用响应面法对超声波辅助提取马钱子中生物碱的工艺进行了优化,在单因素试验的基础上,选择提取溶剂中乙醇体积分数、超声功率、提取时间为随机因素,进行三因素三水平的Box-Behnken中心组合设计,采用响应面法(RSM)分析了3个因素对响应值的影响。结果表明,超声波法提取马钱子中生物碱的最佳工艺条件为:马钱子颗粒度为20～40目,溶剂中乙醇体积分数为32.3%,溶剂pH值为5,超声功率300W,提取时间为37min,液料比为10:1。在此条件下,士的宁、马钱子碱的提取量分别达到18.75、9.24mg/g,与理论预测值基本吻合,说明该优化方法可行。Response surface methodology （RSM） was applied to optimize the ultrasonic-assisted extraction conditions of alkaloids from Semen Strychni. Based on the signal factor test, ethanol concentration, ultrasonic power and extraction time were chosen as casual factors, and a three-factor and three-level Box-Behnken central composite design leading to a set of 15 combinations of these variables was performed to attain the highest weighed value of yields of strychnine and bmcine （their weighed coefficients both were 0.5 ）. The optimal ultrasonic-assisted extraction conditions were determined as follows： particle size of Semen Strychni 20--40 mesh, ethanol concentration 32.3%, pH 5, ultrasonic power 300 W, ultrasonic-assisted extraction time 37 rain, and ratio of liquid to solid 10：1. Under these conditions, the yields of strychnine and brucine were 18.75 mg/g and 9.24 mg/g respectively, which were consistent with the predicted values. These results demonstrate the validity of this extraction process.

关 键 词：马钱子 生物碱 超声提取 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]