反相高效液相色谱法检测不同品种和叶位桑叶中1-脱氧野尻霉素  

RP-HPLC Detection of 1-Deoxynojirimycin in Different Varieties and Sites of Mulberry Leaves

作　　者：魏兆军[1] 蒋磊[1] 周乐春[1] 汪爱民[1] 张松[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2009年第16期258-261,共4页Food Science

基　　金：安徽省教育厅省级重大自然科学基金项目(ZD2008007-2);教育部新世纪优秀人才基金项目(NCET-07-0251);安徽省第四批优秀青年科技基金项目(08040106803)

摘　　要：建立以芴甲氧酰氯(FMOC-Cl)为衍化剂的柱前衍生化-反相高效液相紫外线检测法测定桑叶中1-脱氧野尻霉素(DNJ)含量的方法,并对不同品种、不同叶位桑叶中DNJ含量进行检测。结果表明:以0.05mol/LHCl为溶剂提取桑叶粉中的DNJ,用芴甲氧酰氯(FMOC-Cl)为衍生化试剂,在pH8.50硼酸盐缓冲液条件下,衍生化和RP-HPLC检测效果最好。反相高效液相色谱-紫外检测法检测DNJ时,流动相中乙腈:0.1%醋酸为55:45(V/V)时色谱分离效果最好,DNJ-FMOC的峰面积与DNJ浓度呈高度正相关关系,相关系数为0.9852。不同品种的桑叶中DNJ含量不同,本研究的15个样品中青阳青桑的DNJ含量最高,达到0.1486%,皮桑含量最低,为0.04291%。不同叶位桑叶的DNJ含量不同,第1叶位至第5叶位桑叶的DNJ含量逐渐降低,说明桑叶随着发育成熟,DNJ的含量逐渐降低。结果显示该方法快捷、稳定、灵敏、准确度高,适合桑叶中DNJ含量的检测。A method of pre-column derivatization with FMOC-C1 followed by RP-HPLC with UV detection was established for the determination of 1-deoxynojirimycin （DNJ） content in different varieties and sites of mulberry leaves. DNJ was extracted from mulberry leaves using 0.05 mol/L HCl and derivatized using FMOC-Cl in borate buffer solution （pH 8.50） prior to RP-HPLC detection. The chromatographic separation was achieved on a Waters X-Terra column using a mobile phase consisting of a mixture of acetonitrile and 0.1% aqueous acetic acid （55：45, V/V）. There was a high positive correlation between the peak area and concentration of DNJ-FMOC with a correlation coefficient of 0.9852. The DNJ content was varied in different varieties of mulberry leaves. Among 15 varieties of mulberry, Qingyang Qing mulberry leaves contained the highest DNJ （0.1486%）, while Pi mulberry leaves the lowest （0.04291%）. Also, mulberry leaves in different sites contained different DNJ contents. It decreased from the top to bottom gradually, indicating DNJ content is reduced with the maturation of mulberry leaves.

关 键 词：桑叶 1-脱氧野尻霉素 反相高效液相色谱 衍生化 

分 类 号：R284.1[医药卫生—中药学]