genistein后处理对缺血/再灌注损伤的大鼠心肌细胞凋亡和超微结构的影响  

Effect of genistein pharmacological post-treatment on cardiomyocyte apoptosis and ultrastructure of myocardial tissue in rats with ischemia-reperfusion injury

作　　者：燕炯[1] 孔临萍[2] 刘慧[3] 许言午[3] 马靖[4] 尉肖[4] 吴博威[3] 

机构地区：[1]山西医科大学公共卫生学院营养与食品卫生学教研室,太原030001 [2]山西省精神卫生中心 [3]山西医科大学基础医学院生理学教研室 [4]山西医科大学公共卫生学院

出　　处：《山西医科大学学报》2009年第8期680-684,共5页Journal of Shanxi Medical University

基　　金：山西医科大学博士启动基金资助项目(03200711);山西医科大学学生创新基金资助项目(200744);山西省普通高校大学生创新性实验项目(200805)

摘　　要：目的观测genistein后处理对在体大鼠心脏局部缺血/再灌注模型大鼠心肌细胞凋亡、心肌组织病理形态学及超微结构变化的影响。方法40只实验动物随机分为5组,分别为假手术组、缺血/再灌注组、genistein0.1,1.0,5.0mg/kg后处理给药组。光镜下观察各组心肌组织病理变化,电镜下观察各组心肌组织超微结构变化,DNA原位末端缺口标记法(TUNEL)检测各组心肌细胞凋亡数,观察凋亡指数变化,检测各组心肌组织中caspase-3活性,观察caspase-3的比活性变化。结果各剂量genistein后处理组的凋亡指数与缺血/再灌注组相比均显著下降,各组caspase-3比活性变化趋势与凋亡指数变化趋势相吻合。心肌组织损伤的病理形态学和超微结构随给药剂量不同而变化,呈现出1.0mg/kg组损伤最轻,0.1mg/kg时损伤次之,5.0mg/kg时损伤又较重。结论genistein后处理对在体大鼠心肌缺血/再灌注损伤具有保护作用,保护机制与抑制心肌细胞凋亡有关,并且该保护效应与给药剂量相关。Objective To explore the effect of genistein pharmacological post-treatment on the eardiocyte apoptosis, pathomorphology and uhrastruetural changes of myocardial tissue in rats with isehemia-reperfusion injury. Methods Forty rats were randomized into 5 groups：sham group,isehemia-reperfusion group and 0.1,1.0,5.0 mg/kg genistein post-treatment groups. The pathological and the ultrastruetural changes of cardiac muscle were observed under light microscope and electron microscope, respectively. The apoptosis index was determined using TUNEL, and the activity of easpase-3 was detected. Results The apoptosis index and the activity of easpase-3 significantly decreased in genistein post-treatment groups compared with ischemia-reperfusion group. In genistein post-treatment groups, the pathomorphology and ultrastrueture of myoeardium tissue showed the most serious injury in 5.0 mg/kg genistein post-treatment group, and the lightest in 1.0 mg/kg genistein post-treatment group. Conclusion Genistein pharmacological post-treatment has a protective effect on isehemia-repeffusion injury in rats, which may be induced by the inhibition of cardiocyte apoptosis. The protective effect is related to the dosage.

关 键 词：GENISTEIN 缺血/再灌注 药物后处理 凋亡 超微结构 

分 类 号：R361.3[医药卫生—病理学]