丙烯酰胺人工抗原的合成及其多克隆抗体的制备  

Synthesis of Acrylamide Artificial Antigen and Preparation of Anti-Acrylamide Polyclonal Antibody

作　　者：张红星[1] 高美琴[2] 张馨如[1] 刘慧[1] 

机构地区：[1]北京农学院食品科学系,北京102206 [2]华中农业大学食品科技学院,武汉430070

出　　处：《中国农学通报》2009年第16期83-85,共3页Chinese Agricultural Science Bulletin

基　　金：北京市青年骨干教师支持项目;北京农学院青年基金

摘　　要：利用偶联方法合成丙烯酰胺人工抗原,通过免疫方法获得抗丙烯酰胺多克隆抗体;应用戊二醛法将丙烯酰胺与牛血清白蛋白(BSA)进行偶联,并对这两种物质及其复合物进行紫外扫描,并用此复合物免疫兔子,利用间接酶联免疫吸附法测定抗体的效价;应用牛血清白蛋白与丙烯酰胺偶联人工抗体成功,抗体效价大于8100;应用人工抗原免疫成功制备抗丙烯酰胺多克隆抗体。Artificial antigen of acrylamide was synthetized by couple method, and anti- acrylamide polyclonal antibody were obtained by immunizing method. The Glutaraldehyde method was used to conjugate Acrylamide to Bovine Serum albumin（BSA）, and artificial antigen, acrylamide and BSA were respectively taken on UV spectrum scan. Rabbits had been immunized with artificial antigen, and the titer antiserum from those rabbits were testified by indirect enzyme-linked immunosorbent assay. The results showed that artificial antigen was synthesized successfully between Acrylamide and BSA, and titer of anti-acrylamide serum was above 8100; Anti-Acrylamide polyclonal antibody was prepared by immunizing method of artificial antigen.

关 键 词：丙烯酰胺 人工抗原 多克隆抗体 

分 类 号：TS201.2[轻工技术与工程—食品科学]