灭活原生质体融合技术提高豆鼓纤溶酶菌产酶量  

Enhancing activity of fibrinolytic enzyme-producing strain by protoplast fusion of inactivated parents

作　　者：李灿明[1] 黄时海[1] 张云开[1] 黄珊[1] 齐辉连[1] 陈桂光[1] 覃拥林[1] 梁智群[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005

出　　处：《食品工业科技》2009年第8期140-142,共3页Science and Technology of Food Industry

摘　　要：以豆豉纤溶酶产生菌株蜡状芽孢杆菌UV-101和MW-101作为亲本菌株,首先对亲本原生质体进行紫外灭活,然后用PEG(6000)作为融合剂对灭活双亲进行原生质体融合。通过对再生平板上长出的融合子进行平板初筛和摇瓶复筛,最终获得一株高产菌株PF-101,其酶活比亲本菌株分别提高了74·47%和86·36%,并且该菌株具有良好的遗传稳定性。The protoplasts of Bacillus cereus UV-101 and MW-101 were inactivated by ultraviolet ray, and then protoplast fusion with PEG （as fluxing agent）was conducted on them.A high-yield strain PF-101 was obtained from fusants by first screening of flat plate and second screening of shake flask.Its fibrinolytic activity was increased by 74.47 % fold and 86.36% fold compared with its parents UV-101 and MW-101, respectively.And it can descend stably after five aeneration.

关 键 词：原生质体 灭活 融合 纤溶酶 

分 类 号：TS201.1[轻工技术与工程—食品科学]