产Macrolactin A抗生素海洋解淀粉芽孢杆菌的鉴定及发酵条件优化  

Identification of A Macrolactin A Antibiotic-producing Marine Bacillus amyloliquefaciens JY-863 Strain and Optimization of Its Fermentation Conditions

作　　者：杨桥[1] 韩文菊[1] 张文俊[2] 卢小玲[1] 刘小宇[1] 许强芝[1] 焦炳华[1] 

机构地区：[1]第二军医大学生化与分子生物学教研室,上海200433 [2]上海海洋大学食品学院,上海201306

出　　处：《药物生物技术》2009年第4期311-315,346,共6页Pharmaceutical Biotechnology

基　　金：国家"863"计划重大项目(2007AA091501);中国博士后科学基金资助项目(20080431365)

摘　　要：为筛选海洋微生物来源的抗生素资源,从我国东海海泥中分离到一株可产大环内酯抗生素Mac-rolactinA(MLA)的海洋细菌,经形态特征、生理生化特性及16SrRNA测序及比对、细胞脂肪酸成分分析等多项指标测定,鉴定并将其命名为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)JY-863。利用Plackett-Burman设计及响应面法对影响该菌株发酵产MLA的主要因素进行了优化。确立其最适发酵条件为:初始pH值6,温度29.9℃,装液量52.3%,转速130r/min,接种量10%,培养时间7d,在优化发酵培养条件下,产生的MLA含量提高了5倍,达到18.5μg/ml。To seek for the marine microbe-derived antibiotics resource, a macrolaetin A antibiotic-producing marine bacteria were isolated from the mud sampled at 30 m deep in the East China Sea. This strain was identified and nominated as Bacillus amyloliquefaciens JY- 863 based on the taxonomic studies including phenotypic, 16S rRNA gene sequencing and blast analysis, and fatty acid components analysis. Plackett-Burman design and response surface methodology were applied on the optimization of the fermentation conditions for the increase of macrolactin A production fermented by JY - 863 strain. The fermentation conditions were optimized as follows： temperature 29.9℃, initial pH value 6.0, medium volume 52.3%, rotation speed 130 r/min, inoculation 10% and fermented for 7 days. Under the optimized conditions, the concentration of macrolactin A was increased five-folds and reached 18. 5μg/ml.

关 键 词：Macrolactin A抗生素 鉴定 发酵 PLACKETT-BURMAN设计 响应面法 

分 类 号：TQ92[轻工技术与工程—发酵工程]