乳腺癌HER-2基因扩增和蛋白表达与ER、PR的关系及临床意义  

作　　者：李霄阳[1] 罗定存[3] 曾海鹰[1] 徐雪琴[2] 谢丙乐[2] 唐少华[2] 

机构地区：[1]温州医学院定理临床学院肿瘤外科,325000 [2]温州医学院定理临床学院中心实验室,325000 [3]杭州市第一人民医院,310006

出　　处：《浙江临床医学》2009年第8期787-790,共4页Zhejiang Clinical Medical Journal

摘　　要：目的探讨HER-2基因及其蛋白在乳腺癌中扩增及表达状况，并研究其与ER、PR的相关性。方法用FISH技术和IHC技术检测51例乳腺癌新鲜标本中的HER-2基因的扩增及蛋白表达情况，并检测ER、PR进行对比分析。结果51例中10例HER-2蛋白表达强阳性（＋＋＋），6例阳性（＋＋），2例弱阳性（＋），33例阴性，其中基因过表达率（IHC检测＋＋／＋＋＋）为31．4％；FISH检测中基因扩增14例，37例无扩增，扩增率为27．5％。IHC（＋＋／＋＋＋）与FISH检测符合率87．5％（P〈0．05），ER、PR表达情况与HER-2基因及蛋白扩增呈负相关，ER和PR均为阴性的HER-2基因扩增率及蛋白表达率明显高于ER和（或）PR阳性的患者（IHC50％与24．3％，P〈0．05；FISH42．9％与19．4％，P〈0．05）。结论HER-2的扩增和蛋白过度表达提示乳腺癌预后不良，HER-2与ER、PR在乳腺癌的治疗中起重要作用，IHC可以作为检测HER-2基因状态的首选方法，但对于HER-2（＋＋）需要进一步进行FISH检测。Objective To study the amplification and expression of HER -2 gene and proteinum in breast cancer and to investigate the relationship between HER - 2 and proteinum and ER, PR. Method Apply fluorescence in situ hybridization and immunohistochemistry method to detect the amplification and expression of HER - 2 gene and proteinum and the expression of ER, PR in 51 case of breast cancer. All analysis were carried out using SPSS software. Results HER - 2 overexpression was 16 （31.4%） ,with（ ＋ ＋ ＋ ） 10 cases and （ ＋ ＋ ） 6 cases, There are 14 cases to find HER -2 gene amplification,the positive rate was 27. 5 % , the coincidence of HER -2 overexpression and HER -2 gene amplification was 87.5% （P 〈0. 05 ） , the expression of HER -2 had negative correlation with the expression ER ,PR, HER- 2 gene and proteinum positive expression was significant higher in PR and ER negative group than in PR and （or） ER positive group （ IHC 50% VS24. 3% ,P 〈0. 05, FISH 42.9% VS19.4% ,P 〈 0. 05 ）. Conclusion The amplification and overexpression of HER - 2 gene and proteinum predict unfavourable prognosis in breast cancer. ER, PR and HER -2 play important roles on the occurrence and development in breast cancer. IHC can be used as a preferred method for detecting HER -2 gene, but for HER -2 （ ＋ ＋ ）, further FISH test is needed.

关 键 词：HER-2癌基因 荧光原位杂交 免疫组化 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤] R735.9[医药卫生—临床医学]