两种不同的染色方法评价大鼠心肌缺血-再灌注损伤程度  

Assessment of myocardial ischemia-reperfusion injury with two different staining methods

作　　者：杨帆[1] 赵文来[2] 米琼宇[1] 谢利平[1] 刘振[1] 张雯[1] 李晓珍[1] 黄艳[1] 季勇[1] 

机构地区：[1]南京医科大学动脉粥样硬化研究中心,江苏南京210029 [2]南京市胸科医院心内科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2009年第8期1055-1058,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展重点项目(06NMUZ006);南京市卫生局医学科技发展课题资助项目(YKK08137)

摘　　要：目的:探讨评估心肌缺血-再灌注损伤程度的两种染色方法。方法:大鼠冠状动脉左前降支结扎缺血30min,再灌注6h形成缺血-再灌注损伤动物模型。超声检测心功能指标,并分别用TTC单染法和Evans blue-TTC双染法评价心肌的损伤程度。结果:Evans blue-TTC双染法可以评价出心肌的缺血组织面积、梗死组织面积和正常组织面积,而TTC单染法只能测出组织的梗死面积和缺血面积,不能排除由于结扎位置不同而引起的误差。同时,双染法得出的梗死面积/危险面积的比值(I/R)与心功能指标的相关性,比单染法得出的梗死面积/左心室的面积比值(I/T)与心功能指标的相关性更高。结论:Evans blue-TTC双染法比TTC单染法能更客观更准确的反映出心肌组织缺血-再灌注损伤的程度。Objective：To discuss two different ways of staining methods in evaluating myocardial ischemia-reperfusion injury. Methods： Anesthetized SD rats were subjected to myocardial ischemia reperfusion injury （ischemia 30 mins/reperfusion 6 hrs）. Hemodynamic parameters were measured upon the completion of reperfusion in rats. The hearts were stained with TTC or Evans btue-TFC double-staining, separately, to determine the extent of myocardial damage. All the rats used in the experiment had the same LV morphology and the similar function. Results：The Evans blue-TIC double-staining method can measure the ratio of infarct areas- to-total left ventricular areas （I/R ratio）,While the TIC staining can only measure the ratio of infarct areas-to-risk areas （I/T ratio）. Compared with the I/T ratio,the I/R ratio has higher relevance with the cardiac function. Conclusion：The Evans blue-TTC double- staining method can determine the degree of myocardial damage induced by the ischemia-reperfusion injury more objectively and accurately than the TTC staining method.

关 键 词：心肌缺血-再灌注损伤 EVANS blue-TTC染色 梗死面积 

分 类 号：R361[医药卫生—病理学]