结核分枝杆菌特异性抗原的N末端氨基酸序列分析  

N-terminal Amino Acid Sequence Analysis of Specific Antigen of Mycobacterium Tuberculosis

作　　者：刘文[1] 陈佩丽[1] 高大操[1] 庄玉辉[2] 王洪海[1] 陈岳祥 桂晓红 

机构地区：[1]复旦大学 [2]北京解放军309医院 [3]上海生物制品所 [4]上海第一肺科医院

出　　处：《复旦学报（自然科学版）》1998年第4期532-536,共5页Journal of Fudan University：Natural Science

摘　　要：把WesternBlot分析抗原抗体反应的高度特异性与PVDF膜作为载体进行蛋白质氨基酸序列分析的高度敏感性相结合，对结核分枝杆菌的特异性抗原表位进行筛选和分析鉴定，获得了一个分子质量为31ku，N末端为AlaGluValAspTrpLeuValPheAlaValSerTrpProValGly的结核分枝杆菌抗原，与抗结核的单克隆抗体和结核病人血清发生阳性反应．特异性和保护性抗原的筛选为结核病的诊断、治疗及新型疫苗的构建打下了基础．A specific 31 ku antigen of Mycobacterium tuberculosis was selected. and determined in combination of the high specificity of antigen-antibody reaction with the high sensitivity of amino acid sequence analysis on PVDF membrane. N-terminal sequence of the antigen is Ala Gin Val Asp Trp Leu Val Phe Ala Val Ser Trp Pro Val Gly. It has positive reaction with anti-M. tuberculosis MAb and with serum of TB patients. Selection of specific and protective antigens is the basis of tuberculosis diagnosis, theraphy and vaccine design.

关 键 词：结核分枝杆菌 抗原表位 序列分析 结核病 氨基酸 

分 类 号：R520.3[医药卫生—内科学] R392.11[医药卫生—临床医学]