PCR-SSCP研究肝癌原发灶和转移灶中P16^(INK4)exon2的突变  

PCR-SSCP Analyzing Mutation in P16^(INK4)exon2 of Idiopathy and Metastasis of Liver Cancer

作　　者：曹跃琼[1] 王丰[1] 乔守怡[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《复旦学报（自然科学版）》1998年第4期537-541,共5页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金

摘　　要：用两步PCR法，成功地从石蜡包埋组织DNA中扩增了P16INK4exon2片段；并用单链构象多态（SSCP）分析PCR产物的差异．在32例肝癌原发灶中，发现P16INK4exon2缺失突变6例、SSCP可检出突变1例；在48例肝癌转移灶中，发现P16INK4exon2缺失突变11例、SSCP可检出突变2例．The P16INK4exon2 has been successfully amplifed by two-step-PCR from tissues embeded in parraffin, and the differences of the PCR products were analyzed by SSCP. Six deletions detected by PCR and one SSCP-detectable mutation were found in 32 idiopathy liver cancers while eleven deletions detected by PCR and two SSCP-detectable mutations in 48 liver cancers.

关 键 词：肝癌 原发灶 转移灶 PCR SSCP DNA 

分 类 号：R735.7[医药卫生—肿瘤]