抗克伦特罗重组抗体发酵条件的优化  

Optimization of fermentation of anti-clenbuterol single chain Fv recombinant by E.coli

作　　者：梁艳[1,2] 王弘[2] 杨金易[2] 刘细霞[2] 徐小艳[2] 张宏斌[3] 雷红涛[2] 沈玉栋[2] 孙远明[2] 

机构地区：[1]广州市质量监督检测研究院,广东广州510110 [2]广东省食品质量与安全重点实验室/华南农业大学食品质量安全研究所,广东广州510640 [3]广州军区广州总医院,广东广州510010

出　　处：《广东农业科学》2009年第8期200-202,共3页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30871755&30400354);国家"863"计划(2007AA10Z437);广东省自然科学基金(06025825)

摘　　要：为提高抗克伦特罗(clenbuterol,CBL)单链抗体(single chian Fv,scFv)的表达量,考察了Mg2+、Ca2+、NH4+、NaHPO4-等无机离子,初始pH值以及装液量对重组抗体表达量的影响。优化试验结果表明,重组大肠杆菌E.coli BL21在初始pH值为7.2、含2 mmol/L Ca2+和20 mmol/L NaHPO4-的培养基中培养,装液量为25 mL,42℃诱导表达后抗CBL-scFv的表达量为33.7%。The fermentation process of recombinant anti-clenbutero （CBL） single chain Fv （scFv） was studied in order to improve the expression level of recombinant protein. The effects of factors such as, concentration of inorganicions, i. e. Mg^2＋ , Ca^2＋, NH4^＋ and NaHPO4^-, pH ,and volume of medium were investigated. The results showed that the expression level of anticlenbuterol scFv was increased to about 33.7% of the total bacteria protein when recombinant E.coli was induced at 42℃ with pH 7.2, 25 mL volume of medium with 2 mmol/L Ca^2＋ and 20 mmmol/L NaHPO4^-.

关 键 词：克伦特罗 重组抗体 发酵 

分 类 号：Q786[生物学—分子生物学]