白细胞介素-6对猪脂肪细胞脂肪分解的影响  

Effect of Interleukin-6 on Lipolysis in Porcine Adipocytes

作　　者：杨永青[1,2] 杨公社[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌712100 [2]山西师范大学生命科学学院,临汾041000

出　　处：《畜牧兽医学报》2009年第8期1131-1138,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10Z138);国家重点基础研究发展计划项目(973计划)(2004CB117500)

摘　　要：为了研究白细胞介素(Interleukin,IL)-6对猪脂肪细胞脂肪分解的影响及其分子机制,分化的猪脂肪细胞用不同浓度的IL-6(0、25、50、75、100 ng.mL-1)处理24或48 h。通过测定培养液中的甘油浓度检测脂肪细胞的脂解率;采用形态学观察检测脂肪细胞中甘油三酯积聚量的变化;分别用RT-PCR和Western blot检测脂肪细胞中perilipin A、PPARγ2(Peroxisome proliferator-activated receptor-gamma2)、HSL(Hormone-sensitive lipase)和AT-GL(Adipose triglyceride lipase)的mRNA及蛋白表达。结果显示,IL-6以剂量和时间依赖性的方式刺激猪脂肪细胞的脂肪分解,同时PPARγ2和perilipin A的mRNA及蛋白表达被显著下调;HSL的mRNA表达显著上调,但其蛋白表达水平并未发生显著改变;ATGL的mRNA和蛋白表达均未发生显著改变。上述研究结果表明,IL-6通过抑制PPARγ2及其靶基因perilipin A的表达直接刺激猪脂肪细胞的脂肪分解。The purpose of the study was to explore the effect of interleukin （IL）-6 on lipolysis in porcine adipocytes and the underlying molecular regulation mechanism. Porcine primary adipocytes were cultured and incubated with different concentrations of IL-6 （0, 25, 50, 75 and 100 ng·mL^-1） for 24 or 48 h. Then, the glycerol released into the media was measured as an indicator of the lipolysis. Lipid accumulation morphology was visualized by phase-contrast microscopy. Further, gene expressions of perilipin A, peroxisome proliferator-activated receptor-gamma2 （PPAR γ2）, hormone-sensitive lipase （HSL） and adipose triglyceride lipase （ATGL） were analyzed with semi-quantitative RT-PCR, and their protein level were determined with Western blot. The results showed that IL-6 stimulated lipolysis in differentiated porcine adipocytes in a dose- and time-dependent manner. Meanwhile, the mRNA and protein level of PPAR γ2 and perilipin A were significantly reduced. Although the expression abundance of HSL mRNA was significantly elevated, its protein level had no marked changes. The mRNA and protein level of ATGL were not affected. These findings imply that chronic high dose of IL-6 directly stimulates lipolysis in porcine adipocytes through repressing PPAR γ2 and its target gene perilipin A.

关 键 词：白细胞介素-6 猪脂肪细胞 脂肪分解 PERILIPIN A 

分 类 号：S828[农业科学—畜牧学] Q343.6[农业科学—畜牧兽医]