细支气管肺泡癌和肺腺癌EGFR基因扩增的研究  

The Study on Gene Amplification of EGFR in Bronchioloalveolar Carcinoma and Conventional Adenocarcinoma of the Lung

作　　者：宋欣[1] 宋志刚[1] 吕亚莉[1] 钟梅[1] 李向红[2] 

机构地区：[1]中国人民解放军总医院病理科,北京100853 [2]北京大学临床肿瘤学院、北京肿瘤医院暨北京市肿瘤防治研究所病理科,北京100142

出　　处：《中国肺癌杂志》2009年第8期879-883,共5页Chinese Journal of Lung Cancer

基　　金：中国人民解放军全军医药卫生科研基金项目(No.06MA253)资助~~

摘　　要：背景与目的分子靶向治疗药物表皮生长因子酪氨酸激酶抑制剂(EGFR-TKI)可提高肺腺癌病人的生存率,不同组织类型疗效不同。本研究对细支气管肺泡癌和经典型肺腺癌中表皮生长因子受体(EGFR)基因扩增情况进行比较分析,为临床预后治疗提供依据。方法根据2004年WHO最新肺肿瘤分类标准选取2004年1月-2006年12月期间中国人民解放军总医院病理科病理档案中细支气管肺泡癌(BAC)27例(男13例、女14例)、经典型肺腺癌23例(男11例、女12例),具有BAC成分的混合亚型肺腺癌39例(男18例,女21例),总计89例。应用组织芯片和荧光原位杂交技术(FISH)进行EGFR基因扩增的检测。结果在经典型肺腺癌病例中EGFR基因的扩增率为45.45%,BAC病例中的EGFR基因的扩增率为14.81%,混合亚型肺腺癌为22.58%。经典型肺腺癌的扩增病例明显多于BAC和混合亚型肺腺癌,三组之间c2=11.632,P<0.05。三组病例中EGFR基因扩增多以散在信号存在。结论EGFR基因在肺腺癌的扩增率高于BAC。Background and objective Patients with adenocarcinoma of the hing have disproportionately response to the epidermal growth factor receptor tyrosine kinase inhibitor （EGFR-TKI）. The aim of this study is to analyze the difference of EGFR gene amplification in bronchioloalveolar carcinoma （BAC）, adenocarcinma mixed subtype and conventional adenocarcinoma of the lung and provide some information to clinical therapies. Methods Lung cancer cases were collected and reviewed from the archives of the Department of Pathology, Chinese PLA General Hospital during the time period from 2004 to 2006. The definite diagnosis of BAC based on 2004 WHO classification of lung tumors was made by two pathologists. Fluorescence in situ hybridization （FISH） was performed to detect EGFR gene amplification in pure BAC, adenocarcinma mixed subtype and conventional adenocarcinoma. Results Conventional adenocarcinoma had higher EGFR amplification compared with pure BAC and adenocarcinma mixed subtype （χ^2=11.632, P〈0.0S）. EGFR gene amplification was found in 45.45% of conventional adenocarcinoma, 14.81% in pure BACs, and 22.58% in adenocarcinma mixed subtype. EGFR gene amplification was observed as scattered signals in most cases. Conclusion EGFR gene amplification was seen more frequently in the invasive components than in BAC. EGFR gene amplification might be associated with the development of adenocarcinoma of the lung.

关 键 词：细支气管肺泡癌 肺腺癌 EGFR FISH 组织芯片 

分 类 号：R734.2[医药卫生—肿瘤]