磷酸盐缓冲液法提取茶叶多酚氧化酶的工艺研究  

Reasearch on Extraction of Polyphenol oxidase from Fresh tea with Phosphate Buffer

作　　者：王丽霞[1] 黄文成[1] 林启训[2] 陆则坚[2] 

机构地区：[1]漳州师范学院生物科学与技术系,福建漳州363000 [2]福建农林大学食品科技学院,福建福州350002

出　　处：《淮阴师范学院学报（自然科学版）》2009年第2期166-168,172,共4页Journal of Huaiyin Teachers College;Natural Science Edition

基　　金：福建省自然科学基金资助项目(B9910010)

摘　　要：采用磷酸盐缓冲液提取法,研究了从茶鲜叶中提取多酚氧化酶的最佳工艺条件.结果表明,茶鲜叶多酚氧化酶提取的最佳工艺条件为pH 6.4,料液比(茶叶g∶缓冲液mL)为1∶1,离心转速为9000 r/min,离心时间35 min.The optimum conditions of extracting Polyphenol oxidase from the fresh leaves of tea were studied with Phosphate Buffer. The results show that the optimum extraction technique of Polyphenol oxidase was as pH 6.6,the ratio 1 ： lfor fresh leaf （g）to buffer solution （mL）, 9000 r/min for the centrifugal rotational speed and the centrifugal time with 35min.

关 键 词：磷酸盐缓冲液 茶 多酚氧化酶 

分 类 号：S571.101[农业科学—茶叶生产加工]