无溶剂体系中复合酶催化大豆油水解的研究  

Compound lipase catalyzed hydrolysis of soybean oil in a solvent-free system

作　　者：黄健花[1] 刘亚轩[1] 金青哲[1] 刘元法[1] 王兴国[1] 

机构地区：[1]江南大学食品学院食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《中国油脂》2009年第9期55-59,共5页China Oils and Fats

基　　金：国家"十一五"支撑计划项目(20060BAD27B03);教育部博士点基金项目(20070295004)资助

摘　　要：研究了无溶剂体系中复合酶催化大豆油水解反应。对Sigma L-3126、PPL、C.lipolytica(CLL)、Novo435和Amano-G 5种不同来源的脂肪酶进行筛选和复合使用,得到了水解效果较好的CLL和Amano-G组合。超声波对复合酶反应有一定的促进作用,在反应开始阶段其作用较为明显;较好的反应条件为:CLL和Amano-G的加入量分别为1%和0.1%,在CLL反应1 h后加入Amano-G,反应温度45℃,去离子水直接加入反应体系作为水相,水油质量比1∶1。在此条件下反应24 h水解率由常规振荡水浴的45.7%提高到94.2%。Reaction conditions for compound lipase catalyzed hydrolysis of soybean oil in a solvent - free system were investigated. Five different source lipases of C. lipolytica（CLL）, Sigma L-3126, PPL, Novo435 and Amano - G were screened by investigating the complex effect. Combining CLL and Amano- G could give a better hydrolysis rate. The hydrolysis reaction could be accelerated by ultra- sound, especially at the initial reaction stage. The optimum reaction conditions were obtained as follows： the dosage of CLL and Amano - G was 1 % and 0. 1%, respectively; Amano - G was added after CLL acted for 1 h ; reaction temperature was 45℃ ; water - phase was de - ionized water, and water/oil ratio was 1 ： 1. Under above conditions, the hydrolysis rate increased from 45.7% in conventional shaking bath to 94. 2% after reaction for 24 h .

关 键 词：复合脂肪酶 大豆油 水解 无溶剂体系 

分 类 号：TQ641[化学工程—精细化工]