RGDS—GG—KLAKLAKK多肽对人骨肉瘤细胞生长的影响  

Effect of RGDS-GG-KLAKLAKK peptides on the human osteosarcoma cells growth

作　　者：张敏[1] 杨大志[2] 钟森[3] 高飚[4] 

机构地区：[1]湖北省医学会,武汉430064 [2]华中科技大学同济医学院附属协和医院骨科 [3]湖北省十堰市妇幼保健院 [4]孝感市中心医院骨科

出　　处：《中华实验外科杂志》2009年第9期1195-1197,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察多肽RGDS—GG—KLAKLAKK对体外培养人骨肉瘤细胞MG-63生长的影响，并探讨其作用机制。方法将不同浓度（0、25、50、75、100umol/L）多肽RGDS—GG—KLAKLAKK与MG-63细胞混合培养，用噻唑蓝（MYr）比色法测量多肽RGDS—GG—KLAKLAKK对MG-63细胞生长的抑制作用，流式细胞术（FCM）检测多肽RGDS—GG—KLAKLAKK作用于MG-63细胞后的凋亡率，逆转录一聚合酶链反应（1it—PCR）测量MG-63细胞Survivin mRNA水平的变化。结果多肽RGDS—GG—KLAKLAKK明显抑制MG-63细胞的增殖，并有时间和剂量依赖性，在24、48h的IC50分别为46．8、37．6umol／L（P〈0．05）。多肽RGDS—GG—KLAKLAKK随着剂量的增加MG-63细胞的凋亡率从5．23％上升至38．90％（P〈0．01）。MG-63细胞在多肽RGDS—GG—KLAKLAKK处理后，Survivin mRNA的表达出现明显下降。结论多肽RGDS-GG-KLAKLAKK能够抑制人骨肉瘤MG-63细胞的增殖，并诱导其凋亡。Objective To investigate the effects of the peptides （RGDS-GG-KLAKLAKK） on the growth of human osteosarcoma cells （MG-63）. Methods The MG-63 cells were co-cultured with different concentrations of RGDS-GG-KLAKLAKK （ 0,25,50,75,100 umol/L）. The suppressive effect of RGDS-GG-KLAKLAKK on MG-63 cells was examined by MTT assay. The apoptosis of MG-63 cells was observed by flow cytometry （ FCM ）. The Survivin mRNA levels were detected by RT-PCR. Results RGDS-GG-KLAKLAKK obviously suppressed the proliferation of MG-63 cells in both time-and dose-dependent manners, and the IC50 was 46.8,37.6umol/L at 24 and 48 h respectively （ P 〈 0.05）. The FCM results indicated that apoptosis ratio of MG-63 cells was increased from 5.23% of untreated cells to 38. 90% of treated cells （P 〈 0.01 ）. RT-PCR revealed that the expression of Survivin mRNA in MG-63 cells was down-regulated. Conclusion The RGDS-GG-KLAKLAKK can suppress the proliferation of MG-63 cells,and induce their apoptosis.

关 键 词：多肽 骨肉瘤 SURVIVIN 

分 类 号：R738.1[医药卫生—肿瘤] R737.310.2[医药卫生—临床医学]