壳聚糖基温敏水凝胶对牙周常见致病菌的抑制作用  

An in vitro evaluation of the antibacterial activity of chitosan-based thermosensitive hydrogel against periodontal pathogens

作　　者：吉秋霞[1] 邓婧[1] 于新波[1] 徐全臣[1] 许晓燕[1] 

机构地区：[1]青岛大学医学院附属医院口腔科,山东青岛266003

出　　处：《上海口腔医学》2009年第4期397-400,共4页Shanghai Journal of Stomatology

基　　金：山东省卫生厅青年基金(2007QZ021);青岛市科技发展计划(06-2-2-6-nsh-2)~~

摘　　要：目的:评价壳聚糖-壳聚糖季铵盐/甘油磷酸(CS-HTCC/GP)温敏水凝胶对3种牙周常见致病菌的体外抑菌活性。方法:采用琼脂扩散法测定CS-HTCC/GP温敏水凝胶对牙龈卟啉单胞菌、中间普氏菌和伴放线放线杆菌的最小抑菌浓度(MIC)和抑菌环直径。采用SPSS13.0软件包对数据进行t检验。结果:CS-HTCC/GP温敏水凝胶对牙周常见致病菌—牙龈卟啉单胞菌、中间普氏菌和伴放线放线杆菌均有较强的抑菌活性。与抗菌剂联合后,无论是水凝胶基质还是抗菌剂,抑菌活性均明显增强。结论:CS-HTCC/GP温敏水凝胶不仅作为药物载体参与组成局部药物释放系统,同时作为活性因子参与抑菌过程。PURPOSE：To evaluate the in vitro antibacterial activity of chitosan-quaternized chitosan/α,β-glycerophosphate（CS-HTCC/GP） thermosensitive hydrogel against three periodontal pathogens-P.gingivalis,P.intermedia,and A.actinomycetemcomitans.METHODS：An agar diffusion method was used to assess the antimicrobial property of CS-HTCC/GP thermosensitive hydrogel with minimum inhibitory concentration（MIC） and inhibitory zone measurement.SPSS13.0 software package was used for Student’s t test.RESULTS：Three periodontal pathogens strains were all susceptible to CS-HTCC/GP thermosensitive hydrogel.Both matrix of thermosensitive hydrogel and antibiotic exhibited stronger antibacterial activity especially when they were combined.CONCLUSIONS：CS-HTCC/GP thermosensitive hydrogel is not only as the vehicle of antibiotics which joins the local drug delivery system but as an activator which takes part in the antibacterial process.

关 键 词：壳聚糖 温敏水凝胶 抑菌活性 牙周致病菌 

分 类 号：R781.4[医药卫生—口腔医学]