海洋胶原肽改善老年记忆作用试验研究  

Preventive Effects of Marine Collagen Peptide on Learning and Memory in Aged C57BL/6J Mice

作　　者：裴新荣[1,2] 杨睿悦[1] 赵海峰[1] 李琼[1] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100191 [2]首都医科大学食品药品安全评价中心,北京100069

出　　处：《科技导报》2009年第16期26-31,共6页Science & Technology Review

基　　金：国家"十一五"科技支撑项目(2006BAD27B08)

摘　　要：为探讨海洋胶原肽(MCP)对老年学习记忆的改善作用及其机制,选用20月龄雌性C57BL/6J小鼠,随机分为老年对照组和3个MCP干预组,分别喂饲添加0、0.22%、0.44%和1.32%MCP的特殊加工饲料,另设3月龄青年对照组,喂饲普通小鼠生长饲料,干预6个月后应用跳台试验和Morris水迷宫试验进行行为学检测;nissl染色观察海马形态学;同时检测各组动物肝脏中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及海马组织中脑源性神经营养因子(BDNF)的表达情况。结果表明,MCP干预后老龄小鼠的空间学习记忆能力和被动回避能力明显提高;0.44%MCP干预组小鼠肝脏SOD活性显著高于老龄对照组,而MDA含量显著降低;0.44%和1.32%MCP干预组小鼠海马区BDNF的表达高于老年对照组。各组动物海马神经元数量无明显改变。因此,海洋胶原肽具有预防年龄造成的学习记忆能力下降的功能,其作用机制可能是抗氧化活性和促进BDNF的表达。To observe the preventive effects of Marine Collagen Peptide（MCP） on learning and memory in aged mice, 20-month old female C57BL/6J mice were divided into an aged control group and three MCP-treated groups.The effects on cognitive function, hepatic antioxidative systems, hippocampal morphology and expression of Brain-Derived Neurotrophic Factor（BDNF） were studied after feeding the aged mice MCP（0.22% or 0.44% or 1.32%（wt/wt） in diet） for 6 months.Cognitive functions were assessed using the step-down test and the Morris water maze, respectively.Hippocampal morphology was examined by nissl stain.RT-PCR and Western blotwere used to evaluate the expression of BDNF in hippocampus.The results show that their spatial memory and passive avoidance ability were significantly improved when they were fed with MCP.Furthermore, the activity of SOD was significantly increased and the level of MDA was decreased in the 0.44% MCP supplementation group as compared to the aged control group.The expression of hippocampal BDNF was significantly up-regulated in the aged mice treated with 0.44% and 1.32% MCP.However, the number of nisslpositive cell saw no significant difference among all groups.These results suggest that MCP administration has a preventive effect on memory tasks by lowering oxidative damage and up-regulating expression of BDNF.

关 键 词：生物活性肽 学习记忆 衰老 氧化应激 脑源性神经营养因子 

分 类 号：R151[医药卫生—营养与食品卫生学]