万寿菊花超临界萃取物的抗氧化活性  

Antioxidant Activity of Supercritical CO_2 Extracts from Marigold Flower

作　　者：李大婧[1,2] 吴海虹[1,3] 宋江峰[1,3] 刘春泉[1,3] 刘春菊[1,3] 李志强[1,3] 

机构地区：[1]江苏省农业科学院农产品加工研究所,江苏南京210014 [2]东北林业大学林学院,黑龙江哈尔滨150040 [3]国家农业科技华东(江苏)创新中心农产品加工工程技术研究中心,江苏南京210014

出　　处：《江苏农业学报》2009年第4期894-899,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省科技攻关项目(BE2006319);江苏省博士后科研资助计划项目(0602033B)

摘　　要：采用超临界CO2萃取技术辅以乙醇为夹带剂,制备万寿菊花超临界萃取物(Supercritical CO2 extracts of marigold flower,SEMF)。从抗脂质过氧化能力、还原能力及对羟基自由基(.OH)和超氧阴离子自由基(O2-.)的清除能力方面,综合考察SEMF的体外抗氧化活性。结果表明:SEMF对脂质过氧化有较强的抑制能力,并具有较强的还原能力,其清除.OH和O2-.的EC50分别为0.00083 mg/ml、0.00074 mg/ml(以叶黄素计)。SEMF在这些体系中均表现出较强的抗氧化能力,表明SEMF是一种优良的天然抗氧化剂和自由基清除剂。The extracts from marigold flower were obtained by supercritical CO2 extraction with ethanol modifier. Its antioxidant activities were evaluated by antilipid peroxidation, reducing power, as well as hydroxyl radical （·OH） and superoxide ion radical （O2^-·） scavenging. The supercritical CO2 extracts from marigold flower exhibited strong antioxidant activities to peroxidation and reducing power. EC50 expressed by the supercritical CO2 extracts reflecting scavenging effects on · OH and O2^- · were 0. 000 83 mg/ml,0. 000 74 mg/ml,respectively （caculated in lutein content）. The overall results suggested that the supercritical CO2 extracts would be an excellent natural antioxidant and free radical scavenger.

关 键 词：万寿菊 超临界萃取 叶黄素 抗氧化活性 

分 类 号：S682.1101[农业科学—观赏园艺]