正交法优化微波提取茶多酚的工艺  

OPTIMIZATION OF MICROWAVE EXTRACTION PROCESS OF TP BY ORTHOGONAL METHOD

作　　者：林硕[1] 高学玲[1] 岳鹏翔 岳琳娜[1] 

机构地区：[1]教育部农业部茶叶生物化学与生物技术重点实验室安徽农业大学,安徽合肥230036 [2]大闽食品漳州有限公司,福建漳州363000

出　　处：《食品研究与开发》2009年第9期52-55,共4页Food Research and Development

基　　金：安徽省教育厅自然科学研究项目资助(006KJ041C)

摘　　要：对微波提取工艺方法进行改进,并在利用正交法优化常规水提取茶多酚工艺的基础上,进一步优化了微波提取茶多酚的提取工艺。最终确定:常规水提取的最佳提取工艺为:液固比30:1,温度85℃,时间80min,在此工艺条件下茶多酚提取得率为8.7819%;微波提取最佳工艺为:液固比25:1,微波功率750W,提取时间14min,按此工艺进行了重复性验证试验得到茶多酚的平均提取得率为9.6035%,RSD为0.8580%。结果表明:在各自最佳工艺条件下,微波提取较常规水提取提高了9.3556%,而且大大缩短了提取时间,提取结果稳定性极好。Mictowave extraction process has been improved, and further optimize extraction process of TP by microwave extraction on the basis of the water lixiviation by orthogonal optimization method. The best combined condition of the water lixiviation： liquid-lolid ratio 30：1, extraction temperature 80 ℃, extraction time 80min. Under the optimum conditions, the yield of TP is 8.781 9 %. The best combined condition by microwave extraction： liquid-lolid ratio 25：1, microwave power 750 W, extraction time 14min,Under the opt imum conditions, through duplicated experiment the yield of TP is 9.603 5 % ,RSD is 0.858 0 %. Results： Under the respective optimum conditions, compare to water lixiviation, the yield of TP was enhanced by 9.355 6 % by microwave. Meanwhile, the extraction time was reduced greatly, and the extraction rate of TP is extremely stable.

关 键 词：正交试验 微波 茶多酚 提取工艺 稳定性 

分 类 号：TQ914.1[化学工程]