人胰腺癌组织3、5、7、9和18号染色体部分位点的杂合性丢失  

LOSS OF HETEROZYGOSITY MICROSATELLITE DNA ON CHROMOSOME LOCI 3,5,7,9 AND 18 IN HUMAN PANCREATIC CANCER

作　　者：李伟[1,2] 王殿昌[1,2] 李卫东 王秀琴 郝希山[1,2] 吴 

机构地区：[1]天津医科大学附属肿瘤医院腹部肿瘤科 [2]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院细胞生物室,分子肿瘤学国家重点实验室

出　　处：《中华医学遗传学杂志》1998年第5期274-277,共4页Chinese Journal of Medical Genetics

基　　金：国家"攀登计划";"863计划"资助

摘　　要：目的了解人胰腺癌组织３、５、７、９和１８号染色体部分位点的杂合性丢失的状况。方法选择来自３、５、７、９和１８号染色体不同位置的９对多态性引物，对４５例胰腺癌存档石蜡标本经显微镜下剥离后，以ＰＣＲ－ＳＳＬＰ－银染法检测胰腺癌中这些位点的杂合性丢失。结果胰腺癌组织在１８ｑ２１．１、３ｐ１４．２、３ｐ２１．１和９ｑ２２－３１等处存在较高频率的杂合性丢失。Objective Detecting the loss of heterozygosity in paraffin embedded pancreatic cancer tissues.Methods Analysing loss of heterzygosity (LOH) of microsatellite DNA on chromosome loci 3,5,7,9 and 18 with PCR SSLP silver stain method in pancreatic cancer.Results In 45 sporadic pancreatic cancer samples and their paired control tissue, LOH was detected on site for D18S46(18q21 1,31 0%),D18S474(18q21 1,20 0%),D9S176(9q22 31,20 0%),D3S1234(3p14 2,17 5%),D3S1289(3p21 1,15 7%),D3S1481(3p14 2,4 9%),D7S486(7q22,3 6%),D5S365(5q32,3 2%) and D3S587(3p24 26,2.6%).Conclusion Different percentages of loss of heterzygosity on specific chromosomal regions were found, and the meaning of the results was discussed. Some key genes may play a role in the pathogenesis of pancreatic cancer.

关 键 词：胰腺肿瘤 微卫星DNA 杂合性丢失 

分 类 号：R735.9[医药卫生—肿瘤]