酶法制取1,6-二磷酸果糖的工艺研究  

THE STUDY ON PREPARATION AND PURIFICATION OF FRUCTOSE 1,6 DIPHOSPHATE WITH ENZYMATIC METHOD

作　　者：应汉杰[1] 吕浩[1] 欧阳平凯[1] 

机构地区：[1]南京化工大学生物工程及科学系

出　　处：《化学反应工程与工艺》1998年第3期275-281,共7页Chemical Reaction Engineering and Technology

基　　金：国家"八五"重点攻关项目

摘　　要：主要研究了不同质壁分离剂对酵母细胞的通透性以及酶活的影响和酶法合成ＦＤＰ的工艺条件。针对糖酵解过程中的各中间代谢产物的理化特性不同，采用阴离子交换树脂分离ＦＤＰ，用酒精—媒晶剂体系结晶ＦＤＰ。产品的总收率和纯度分别为７５％～８０％和９９．５％The effect of plasmolysing agents or detergents to cell permeability and enzymatic activity and the technological conditions of synthesizing fructose 1,6 diphosphate were studied. It was separated with anion ion exchange resign and crystallized in ethanoladdition system according to the differences of physical chemical characters of all intraccllular intermediates. The yield and purity of product are 75 %～80 % and 99.5 %, respectively.

关 键 词：FDP 制备 纯化 二磷酸果糖 酶法 果糖二磷酸钠 

分 类 号：TQ929[轻工技术与工程—发酵工程]