高温快速消化与国标消化的凯氏定氮法测定蛋白质含量的比较研究  

Comparative Study of Protein Levels Detected by Thermophilic Tachy-digestion and Encoding Standard Digestion of Kjeldahl Determination

作　　者：宋鹏[1] 赵海岳 佟菲[1] 周晋[1] 张迎媚[1] 刘秀华[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院血液实验室,黑龙江哈尔滨150001 [2]哈尔滨世亨生物工程药业股份有限公司质保部,黑龙江哈尔滨150078

出　　处：《黑龙江医学》2009年第9期649-651,共3页Heilongjiang Medical Journal

摘　　要：目的比较高温消化与国标消化的凯氏定氮法检测蛋白含量的准确性与稳定性有无差异,以达到简化操作、节省时间的目的。方法分别用国标消化与高温消化法对50 g/L蛋白校准液(重复15次)及15份人血白蛋白样品的蛋白含量进行检测。结果高温消化与国标消化法检测蛋白含量结果:蛋白校准液分别为49.91 g/L±0.00 g/L和49.95 g/L±0.00 g/L(t=1.1602,P<0.05);人血白蛋白样品分别为:97.65 g/L±0.55 g/L和97.61 g/L±0.57 g/L(t=0.5762,P<0.05)。两种检测方法的回归分析Y=0.9987X+0.0797,R2=0.9999。高温消化法的蛋白回收率为98.0%。结论高温快速消化的凯氏定氮法检测蛋白含量结果和国标规定方法无差异,但比国标规定方法可以大大缩短工作时间,提高工作效率,值得进一步推广。Objective The protein levels were detected by thennophilic tachy - digestion and encoding standard digestion of Kjeldahl detenninlation respectively to compare the difference in exactitude and constancy of the two method to simplify the operation and save time. Methods The proteins of 15 portions of albumin standard liquid and samples were detected by thermophilic tachy - digestion and encoding standard digestion of Kjeldahl determination respectively. Results The standard fluid, s protein levels detected by thermophilie taehy - digestion and encoding standard digestion of Kjeldahl determination were 49.91 g/L ± 0.00 g/L and 49.95 g/L ± 0.00 g/L（ t = 1.1602, P 〈 0.05） respectively. The sample, s protein levels detected by those were 97.65 g/L± 0.55 g/L and 97.61 g/L ± 0.57 g/L（ t = 0.5762, P 〈 0.05） respectively. The regression analysis formula of detection results of the two methods was Y = 0.9987X ＋ 0.0797, R^2 = 0.9999.The protein recovery rate detected by thermophilic tachy - digestion was 98.0%. Conclusion The detected difference of thermophilic tachy - digestion and encoding standard digestion of Kjeldahl determination had no significance, but the former procedure could shorten operating time and raise work efficiency and deserve further generalization.

关 键 词：凯氏定氮法 蛋白质含量 高温消化 

分 类 号：R972.2[医药卫生—药品]