洗发水对蚕豆根尖细胞遗传毒性的研究  

Genetic Toxicity of Shampoo to Root Tip Cells of Vicia faba

作　　者：张江丽[1] 王淑敏[1] 高永闯[1] 刘爱华[2] 张志达[1] 焦彩荣[3] 

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]廊坊市农科院科研处,河北廊坊065000 [3]廊坊师范学院

出　　处：《环境与健康杂志》2009年第9期812-814,共3页Journal of Environment and Health

基　　金：廊坊市科技局资助项目(2007050282)

摘　　要：目的研究3种不同品牌的洗发水对蚕豆根尖细胞的遗传毒性效应。方法以蚕豆根尖为试验材料,在浓度为0.25%的3种洗发水溶液中分别连续诱变、累积诱变2、4、6、8h,观察并计算其微核率和染色体畸变率,以自来水为对照。结果3种洗发水均可诱发蚕豆根尖细胞产生微核及染色体畸变,分别在连续诱变4h和累积诱变8h时出现最高峰,并表现为良好的剂量-效应关系。累积诱变的作用大于连续诱变,与对照组相比,差异有统计学意义(P<0.05或P<0.01)。结论被测洗发水对蚕豆根尖细胞具有遗传毒性,且具有累积效应。Objective To study the genotoxicity of 3 kinds of different brands shampoo to Viciafaba root tip cells. Methods To take root tip cells of Viciafaba as the subjects, genetic safety tests were conducted on those materials treated with shampoo of a certain concentration （0.25%） for 2-8 h of continual mutagenesis and accumulative mutagenesis respectively, micronucleus and chromosomal aberration was observed and calculated, the rate of tap water was used as the control. Results Three kinds of shampoo could induce root tip cells of Viciafaba to produce micronucleus and chromosomal aberration, the peak was in the continual mutagenesis for 4 h and accumulative mutagenesis for 8 h respectively, which showed dose-effect relationship. There were significant differences between the control group and the experimental groups （ P〈0.05 or P〈0.01）. Conclusion Under the conditions of this experiment, the tested shampoo has significant genotoxicity to Viciafaba root tip cells, and has a cumulative effect of genetic toxicity.

关 键 词：洗发水 蚕豆根尖细胞 有丝分裂 微核 染色体畸变 

分 类 号：R994.6[医药卫生—毒理学]