高寒地区冬小麦东农冬麦1号抗冻蛋白的研究  

Research on Antifreeze Proteins of Dongnongdongmai 1 in High-cold Area

作　　者：王多佳[1] 曾俨[1] 牟永潮[1] 于晶[1] 苍晶[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《麦类作物学报》2009年第5期823-826,共4页Journal of Triticeae Crops

基　　金：东北农业大学创新团队项目(C×2003);东北农业大学科学研究基金项目(2008)

摘　　要：为探究小麦抗冻机理,以东农冬麦1号为试验材料,在田间自然降温条件下,分别在5、0、-10、-20和-25℃胁迫下取分蘖节、根和叶,提取全蛋白进行SDS-PAGE分析并观察冰晶形态。结果表明,低温条件下东农冬麦1号分蘖节的全蛋白中出现7条特异蛋白带,其全蛋白对冰晶有修饰作用,并且随着气温的降低,抗冻能力逐渐增强。分蘖节和根中都在-20℃以后产生了分子量约为26 kD的特异条带;-25℃低温下分蘖节中产生了分子量约为17 kD的特异蛋白,推测其可能是产生修饰冰晶能力的抗冻蛋白。分蘖节和根为产生抗冻蛋白的重要部位。Dongnongdongmai 1 is the winter wheat（Triticurn aestiuum L. ） cultivar that can bear low tem- perature and grow normally in Heilongjiang province. The cuhivar was planted under the field conditions and sampled at different temperatures （ 5 ℃, 0℃, -10 ℃, -20 ℃, -25 ℃）. The samples were separated into leaves, tillering nodes and roots. The total proteins were extracted and studied by SDS-PAGE, respectively. The results showed that the total proteins of tillering nodes had several specific protein bands. The total proteins had the ability to modify the ice crystal, and were identified as antifreeze proteins while the temperature decreasing, the concentrations of antifreeze proteins increased gradually. The results of SDS-PAGE showed a specific band with molecular weight of about 26 kD in both in tillering nodes and roots at lower than -20℃ ; and another different band with molecular weight of about 17 kD in tillering nodes at -25 ℃. We speculated that these two protein peptides maybe the antifreeze proteins modifying the ice crystal, and the tillering nodes and roots were the most important organs to generate antifreeze proteins.

关 键 词：小麦 低温 抗冻蛋白 冰晶 

分 类 号：S512.1[农业科学—作物学] S311