鸡血藤嫩叶愈伤组织诱导最佳培养基的筛选  

Screening of Optimum Inducing Callus culture Media from Tender Leaves of Spatholobus suberectus Dunn

作　　者：吴桂容[1] 陈春岚[1] 曲芬霞[1] 邓年方[1] 邓志勇[1] 刘玉清[1] 何忠伟[1] 

机构地区：[1]贺州学院化学与生物工程系,广西贺州542800

出　　处：《现代农业科技》2009年第18期93-94,共2页Modern Agricultural Science and Technology

基　　金：广西贺州市科学研究与技术开发计划项目资助(贺科攻070402N)

摘　　要：以鸡血藤植物密花豆嫩叶为材料,不同培养基的诱导率为测定指标,筛选出一种适于鸡血藤植物嫩叶愈伤组织诱导的理想培养基,结果发现:优化得到诱导率为95%的最佳培养基,即MS+1.0mg/L 6-BA+0.5mg/L NAA+30g/L蔗糖+0.1g/L VC,此培养基对防止愈伤组织的褐化也有较好效果。Choosing tender leaves of Spatholobus suberectus Dunn. as material,inductivity of different mediums as index, the ideal inducing callus cultrue media fit for tender leaves ofSpatholobus suberectus Dunn was screened. The results showed that the optimum inducing callus cuture media was MS＋ 1.0mg/L 6-BA＋0.5mg/L NAA＋30g/L sucrose＋0.1g/L Vc. The inducfivity of the media was 95%,and it was the best to inhibit the darkening of callus.

关 键 词：鸡血藤 愈伤组织 培养基 筛选 

分 类 号：S567.79[农业科学—中草药栽培]