三孢布拉氏霉菌体中辅酶Q_(10)测定方法的条件优化  

Optimization of determination method of coenzyme Q_(10) from Blakeslea Trispora

作　　者：孟妤[1] 单志萍[1] 

机构地区：[1]江苏省微生物研究所有限责任公司,江苏无锡214063

出　　处：《食品工业科技》2009年第9期162-164,169,共4页Science and Technology of Food Industry

基　　金：江苏省"十五"科技攻关(工业)项目(BF2004031)

摘　　要：为快速测定三孢布拉氏霉发酵制备生产中的辅酶Q10,本研究采用醇碱皂化法,其工艺条件为:KOH加量为干菌体质量的1.9倍,醇碱浓度为10%,焦性没食子酸添加量为湿菌体质量的10%,在70℃下皂化60min。将萃取得到的皂化液采用硅胶HF254为固定相的薄层层析,石油醚(60～90℃):乙醚(20:3)为流动相,分离菌体皂化液中的辅酶Q10。然后在薄板上刮下与辅酶Q10标准品相同Rf值的色带,溶于无水乙醇中,用紫外分光光度法测定其辅酶Q10含量,其回收率达82.8%(RSD值为1.49%)。同时结合高效液相色谱法,确证三孢布拉氏霉菌体中辅酶Q10的存在及含量。The method of alcohol-alkaline saponification was used to fast measure coenzyme Q,o from products of Blakeslea Trispora fermentation, and the technological conditions were as follows： amounts of KOH was 1.9 times compared to dried biomass and pyrogallic acid was 10% compared to wet biomass, respectively; the concentration of alcohol-alkaline was 10%.Saponification was performed for 60min in 70% and coenzyme Q10 was extracted by thin layer chromatographic analysis using silica gel HF254 as fixed phase and benzine （60-90℃）/ ether （20/3） as fluid phase.After then, the inked bands as the same size of Rt to standard coenzyme Q10 were scraped and dissolved in the absolute ethyl alcohol to determine the concentration of coenzyme Q10 by the ultraviolet spectrophotometric method. The recovery percent reached 82.8% （the RSD value was 1.49%）. Simultaneously the properties and quantities of coenzyme Q10 of Blakeslea Trispora were confirmed by the highly effective liquid phase chromatography.

关 键 词：三孢布拉氏霉 辅酶Q10 皂化萃取 薄层-紫外分析法 

分 类 号：TS201.3[轻工技术与工程—食品科学]