一株抗G^-菌和酵母菌的乳酸乳球菌的分离鉴定与抗菌活性  

Identification and Characterization of a Lactococcus lactis Strain with the Distinctive Antimicrobial Activity Against Gram-negative Bacteria and a Yeast

作　　者：黄新凤[1] 江梦天[1] 邵小虎[1] 李小华[1] 李林[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,湖北武汉430070

出　　处：《微生物学通报》2009年第9期1356-1362,共7页Microbiology China

基　　金：国家自然科学基金(No.30670054;30370026)

摘　　要：以G+菌金黄色葡萄球菌(Staphylococcus aureus)作为指示菌,通过抑菌筛选法从生牛奶中初筛得到具有抑菌活性的14株细菌菌株,然后通过个体形态与培养特征观测、部分生理生化反应、G+C mol%测定、16S rDNA序列比对分析、PCR扩增特异性N-乙酰胞壁酸水解酶基因和序列对比分析等鉴定,确定其中的一株具有较高抑菌活性的分离株为乳酸乳球菌乳酸亚种(Lactococcus lactis subsp.lactis)菌株,命名为MB191。对多种G+细菌、G-细菌、酵母菌和丝状真菌的对峙培养抗性测定结果表明,MB191除对供试G+细菌具有较高的抑菌活性以外,还对丁香假单胞菌(Pseudomonas syringae)、荧光假单胞菌(P.fluorescens)等G-细菌和汉逊德巴利酵母(Debaryomyces hansenii)等具有明显的抑菌活性。乳酸乳球菌的这一特性目前尚未见文献报道。Using the Gram-positive Staphylococcus aureus as the indictor bacterium, fourteen antibacterial strains were initially obtained by the bilayer-media screening method from the raw milk samples, and one isolate was found to exhibit the higher antibacterial activity against the indicator. This isolate was further studied on its individual and cultural morphology features, partial physiological and biochemical reaction activities, G＋C content, the sequence features of the 16S rDNA and the species-specific N-acetylmuraminidase gene （acmA）, consequently, it was identified as the Lactococcus lactis subsp, lactis strain, named as MB 191. An evaluation of the antimicrobial spectra of MB 191 was subsequently performed, it showed the remarkable activities against not only the tested Gram-positive bacteria, but also several Gram-negative bacteria including Pseudomonas syringae and R fluorescens, as well as the yeast Debaryomyces hansenii, which was a distinctive feature that was not reported prior to this study.

关 键 词：乳酸乳球菌 鉴定 抑菌活性 革兰氏阴性菌 酵母菌 

分 类 号：Q93[生物学—微生物学]