浓香型大曲原核微生物群落的PCR-SSCP解析  

PCR-SSCP Analysis of the Pronucleus Microbial Communities in Luzhou-flavor Daqu

作　　者：罗惠波[1] 黄治国[1] 李浩[1] 张强[1] 卫春会[1] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000

出　　处：《微生物学通报》2009年第9期1363-1367,共5页Microbiology China

基　　金：四川省科技支撑项目(No.07GG013-004);四川省应用基础项目(No.2006J13-006)资助

摘　　要：采用PCR-SSCP(单链构象多态性)技术对浓香型大曲发酵过程中原核微生物群落结构的变化情况进行了研究,结果表明:(1)发酵各时期曲样的群落结构相似,同时也具有多态性;(2)不同微生物群落具有协同和制约的复杂生态学效应;(3)各时期曲样微生物多样性指数均在1.69～2.01之间,群落结构相对较稳定;(4)各曲样微生物群落相似性位于0.67～1.00之间,邻近时期样品间的相似性程度较高。PCR-SSCP （single-strand conformation polymorphism） was used for studying the community changes of pronucleus microorganisms in various fermenting stages of Luzhou-flavor Daqu. The results showed： （1） The pronucleus microorganism＇s community was similar and also had the polymorphism in each simple of various fermentation stage; （2） Different microflora had complex ecology effects of coordination and the restriction; （3） The diversity indexes of different stage of Daqu microorganisms were around 1.69-2.01, and the composition of them was stable relatively; （4） The similarity indexes were 0.67-1.00, and much higher in the approaching stages.

关 键 词：大曲 PCR-SSCP 16S RDNA 微生物群落 

分 类 号：TS261.11[轻工技术与工程—发酵工程]