微波法选育红曲霉菌种发酵生产生理活性物质的研究  

Study on Microwave Breeding Monascus Ruber and Production of Monacolin K with Fermentation

作　　者：左秀凤[1] 刘隽[1] 杨莉[1] 

机构地区：[1]河南职工医学院医学技术系,河南郑州451191

出　　处：《河南职工医学院学报》2009年第5期441-444,共4页Journal of Henan Medical College For Staff and Workers

摘　　要：目的研究红曲霉菌种发酵生产生理活性物质Monacolin K的条件。方法用微波法选育以红曲霉No.1为出发菌,经50W的微波辐照诱变获得Monacolin K高产菌株,并对其产色素和Monacolin K的发酵条件进行摸索和研究。结果接种剂量3 ml、粳米与豆粕的配比=5∶1、培养温度28℃、培养时间9 d,产品色素含量最高达348μg/g、培养11 d Monacolin K达最大值90μg/g。结论通过微波诱变红曲霉菌种,在适当的发酵条件下,可提高次生代谢物Monacolin K的产量,红曲色价变化不大。Objective The conditions were studied to product Monacolin K with Monascus ruber＇s fermentation. Methods A high-production Monacolin K strain was obtained through 50 W 1. 5 min microwave mutation as compared with the original Monascus ruber Nol. The fermentation conditions of the strain to product pigment and Monacolin K were searched and studied. Results The results showed that the content of Monacolin K could reach 90 μg/g fermentation in 11 d, and the content pigment was 348 μg/g under fermentation in 9 d,under the optimum fermentation conditions,the 3 ml, and the ratio of the solid culture medium was made of plump rice and soybean meal 5： 1, temperature 28℃. Conclusion By the way of microwave inducing monascus ruber＇ s fermentation,the yield of Monacolin K can be increased on the appropriate conditions, but the pigment output is immovable.

关 键 词：红曲霉 微波诱变 固态发酵 色素 Monacolin K 

分 类 号：R65[医药卫生—外科学]