应用激光捕获显微切割技术纯化的鼻咽癌蛋白质表达谱的建立  

Establishment of protein expression profile of laser capture microdissection-purified nasopharyngeal carcinoma tissue

作　　者：彭芳[1] 汤参娥[1] 李茂玉[1] 李萃[1] 程爱兰[1] 李峰[1] 张鹏飞[1] 李美香[1] 肖志强[1] 陈主初[1] 

机构地区：[1]中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙410008

出　　处：《中南大学学报（医学版）》2009年第6期481-486,共6页Journal of Central South University ：Medical Science

基　　金：国家"973"项目(2001CB510207);教育部新世纪优秀人才培养计划基金([2007]70);湖南省科技厅重点科研项目(06SK2004)~~

摘　　要：目的:建立鼻咽癌(nasopharyngeal carcinoma,NPC)蛋白质表达谱,为NPC蛋白质组学研究奠定基础。方法:应用激光捕获显微切割(laser capture microdissection,LCM)技术从NPC组织纯化NPC细胞,应用二维凝胶电泳(2-DE)分离LCM纯化NPC细胞的蛋白质,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)鉴定蛋白质,利用生物信息学资源构建NPC的2-DE蛋白质组数据库。结果:建立了NPC蛋白质2-DE参考图谱,2-DE图谱共显示(1312±30)个蛋白质点,共鉴定了427个蛋白质点,包括241个非冗余蛋白质,并构建了NPC的2-DE蛋白质组数据库,这些数据可从本实验室网站(http://www.xyproteomics.org)上进行查询。结论:首次建立了LCM纯化NPC细胞2-DE蛋白质表达谱,为NPC蛋白质组学研究提供了重要的信息和资料。Objective To establish the protein expression map of nasopharyngeal carcinoma（NPC）,and provide a basis for proteomic study of NPC.Methods Laser capture microdissection（LCM） was used to isolate cancer cells from NPC tissues.Two-dimensional gel electrophoresis（2-DE） was used to separate the total proteins of LCM purified NPC cells.Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry（MALDI-TOF-MS） was performed to identify the proteins,and bioinformatics was used to construct the 2-DE database of NPC proteome.Results A 2-DE reference map of NPC was established.On the 2-DE map,a total of（1 312±30） protein spots were detected,and 427 protein spots representing 241 non-redundant proteins were identified.The 2-DE database of NPC proteome was constructed.These data could be accessed at our website（http：//www.xyproteomics.org）.Conclusion A protein expression profile of LCM purified NPC tissues has been established for the first time,which provides useful information and source for proteomic study of NPC.

关 键 词：鼻咽癌 蛋白质表达谱 蛋白质组数据库 激光捕获显微切割 二维凝胶电泳 质谱 

分 类 号：R739.63[医药卫生—肿瘤]