纤维素酶法提取川芎多糖的工艺条件研究  

Study on the Extraction of Polysaccharide of Ligusticum Chuanxiong Hort by Cellulose Enzymatic Method

作　　者：孙显[1] 王维香[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039

出　　处：《西华大学学报（自然科学版）》2009年第5期103-106,共4页Journal of Xihua University:Natural Science Edition

基　　金：四川省科技厅资助项目(2006J13-054)

摘　　要：采用单因素实验和正交实验研究了时间、温度、pH值、酶加量四个因素对提取川芎多糖的影响。得到提取川芎多糖的最佳工艺条件为:纤维素酶0.25%,时间120min,pH值4.0,温度50℃,在此条件下,多糖的提取率为7.26%。The optimal conditions for extracting polysaccharide of Ligusticum chuanxiong Hort by cellulose enzym was investigated through experiment. The influence factors including reaction time, pH value, adding amount of enzyme and reaction temperature were studied by single factor test with orthogonal planning of the experiment. It was concluded that the optimal condition of extraction was as follows ： 0. 25% concentration of cellulose enzyme, pH 4. 0, 120min reaction time and 50℃ reaction temperature. The results obtained show that the extraction rate of polysaccharide under the optimal condition is 7.26%.

关 键 词：纤维素酶 川芎 多糖 提取 

分 类 号：Q539[生物学—生物化学]