高效液相色谱法测定食品中纽甜的含量  

Determination of Neotame in Food by High Performance Liquid Chromatography

作　　者：蔡基智[1] 林杰[1] 廖金华[1] 

机构地区：[1]广东省食品工业研究所广东省食品工业公共实验室,广东广州510308

出　　处：《广东化工》2009年第9期176-176,188,共2页Guangdong Chemical Industry

摘　　要：建立高效液相色谱法测定食品中纽甜的含量。采用AgilentTCC18柱(4.6mm×250mmi.d.5μm),流动相为乙腈—缓冲溶液(体积比为35?65),流速1.0mL/min,二极管阵列检测器(DAD),柱温30℃。结果表明,纽甜质量浓度在5～100μg/mL范围内与峰面积呈良好的线性关系,回收率在90%～99%。方法简便快速,操作稳定,经济环保,适用于食品中纽甜含量的检测。A high performance liquid chromatographic method was developed to determinc of amounts of neotame in food. The column of Agilent TC C18 （4.6 mm×250 mm i.d.5μm） and a mobile phase of acetonitrile-buffer solution（35： 65, V/V） at a flow rateof 1.0 mk/min and column temperature 30℃ with diode-array detector were adopeted. The method was proved tobe linear in the range of neotame from 5～ 100μg/mk. The recovery was 90 %--99 %. Conclusion： The method was simple, fast and stable. It was suitable for quantitative analysis for the amounts of neotame in i-hod.

关 键 词：高效液相色谱法(HPLC) 纽甜 二极管阵列检测器(DAD) C18柱 

分 类 号：O65[理学—分析化学]