非小细胞肺癌患者XRCC1基因多态性对p53基因突变发生的影响  

Influence of common XRCC1 variant alleles on p53 mutations in NSCLC patients

作　　者：李红[1,2] 毛伟敏[1,3] 

机构地区：[1]浙江中医药大学西医系,杭州310003 [2]宿州市立医院肿瘤内科,234000 [3]浙江省肿瘤医院,310003

出　　处：《临床肿瘤学杂志》2009年第9期777-781,共5页Chinese Clinical Oncology

基　　金：浙江省医药卫生科技创新资助项目(320.67)

摘　　要：目的：观察非小细胞肺癌（NSCLC）患者XRCC1基因399位点多态性以及吸烟对p53基因突变发生的影响。方法：运用不对称PCR技术扩增p53基因的第5～8外显子，用聚合酶链反应一单链构象多态性分析法（PCR．SSCP）检测p53基因突变，并对有差异的结果测序验证；运用PCR技术扩增XRCC1基因的第10外显子，从正反两个方向对扩增片段进行DNA测序分析其多态性；同时了解吸烟与它们的关系。结果：38例可手术的NSCLC患者中发现16例有p53基因的突变，发生突变的16例中6例至少携带1个XRCC1-399Gln等位基因，10例为野生基因型XRCC1—399Arg／Arg，用二项分类Logistic回归模型分析XRCC1—399Gln多态性与p53基因突变两者的关系，优势比（OR）为1．536（95％CI：0．376～6．280）；该模型中吸烟与p53基因突变的OR为1．524（95％CI：0．250～9．295）。结论：NSCLC患者中，XRCC1基因399位点多态性和吸烟情况对p53基因突变发生无明显影响。Objective：To investigate the influence of common XRCC1 variant alleles on p53 mutations in NSCLC patiens. nethods：Exon 10 of XRCC1 were amplified by polymerase chain reaction（PCR）. The PC R products were sequenced and analyzed from both sense and antisense directions by the traditional method with chloroform. Exon 5-8 of p53 were amplified by dissymmetry polymerase chain reaction （PCR）, and analyzed p53 mutated by polymerase chain reaction-single strand conformation polymorphism analysis（PCR-SSCP） ,the products were suspected mutated be sequenced from both sense and antisende directions. Results：The patients who p53 gene mutated,6 cases（6/12,50. 0% ）carried at least one XRCC1-399Gln allele,but the wild homozygous genetype XRCC1- 399Arg/Arg was 10 cases（ 10/24,41.7% ）. Analyse by binary logistic： β =0. 429 ,OR = 1. 536（95% CI：0. 376-6. 280） ;The p53 gene mutation among smokers 50. 0% （11/22） vs. nosmokers 31.3 % （5/16） innoncar（ P = 0. 326）. Analyse by binary logistic ：β = 0. 421, OR = 1. 524 （95 % CI：0. 250-9. 295 ）. Conclusion：The XRCC1-399 variant allele and smoker history did not show any effection on the p53 mutation.

关 键 词：非小细胞肺癌 XRCC1 P53 基因 基因多态性 突变 

分 类 号：R734.2[医药卫生—肿瘤]