酶解-压热法制备淮山药抗性淀粉  

MANUFACTURING DIOSCOREA OPPOSITA THUNB RESISTANT STARCH BY ENZYME-AUTOCLAVING METHOD

作　　者：聂凌鸿[1] 侯莉阳[1] 

机构地区：[1]淮阴工学院食品科学与工程系,江苏淮安223003

出　　处：《食品研究与开发》2009年第10期27-31,共5页Food Research and Development

基　　金：江苏省淮安市农业科技计划项目(SN0775);江苏省高校自然科学基础研究项目(07KJD550022)

摘　　要：以淮山药淀粉为原料,通过正交试验研究酶解-压热法制备抗性淀粉的最佳工艺参数。在压热法的最佳工艺基础上,通过使用普鲁蓝酶处理淀粉,使产率大大提高,该法所得的产率最高可达16.47%左右。确定压热处理最佳工艺条件为淀粉乳浓度25%,pH值8.0,121℃压热处理40min,冷藏老化时间为36h。确定制备抗性淀粉的最佳酶作用参数为加酶量4U/g干淀粉,作用温度55℃,作用时间8h。Dioscorea opposita Thunb resistant starch was prepared by enzyme-autoclaving method. An orthogonal trial was conducted and the optimal parameters for preparation of Dioscorea opposita Thunb resistant starch were investigated. Based on the best process condition of autoclaving method, the pullulanase used to produce resistant starch. It was found that through enzymatic treatment the productivity of resistant starch was considerably raised. The highest content of RS can reach 16A7 % .Through the experiment, the better technology parameters of autoclavingmethodwereobtained： theconcentrationofthestarchis25%, pHvalueisS.0, autoclaving time is 40 min at 121℃, and refrigeration time 36 h.The parameters of enzyme-autoclaving method are that the added quantity of the pullulanase is 4 U/g dry starch, the reaction temperature is 55 ℃ and enzymolysis time is 8 h.

关 键 词：淮山药 抗性淀粉 制备 普鲁兰酶 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ645.95[轻工技术与工程—食品科学与工程]