产胆固醇氧化酶重组大肠杆菌的发酵培养基和诱导条件的优化  

Optimization of Culture Conditions for Production of Cholesterol Oxidase Using Recombinant E.coli

作　　者：杨海麟[1] 王长城[1] 张玲[1] 孙艳[1] 白洁[1] 王武[1] 杨建勋[2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]无锡市广播电视大学,江苏无锡214011

出　　处：《食品与生物技术学报》2009年第5期670-674,共5页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2006AA10Z305);工业生物技术教育部重点实验室基金项目(KLI-KF200505)

摘　　要：在摇瓶中对产胆固醇氧化酶重组大肠杆菌的发酵培养基和诱导条件进行了优化,优化培养基为甘油10 g/L,胰蛋白胨10 g/L,酵母粉5 g/L,KH2PO42 g/L,K2HPO44 g/L,Na2HPO4.12 H2O7 g/L,(NH4)2SO41.2 g/L,NH4Cl 0.2 g/L,MgSO4.7 H2O1 g/L;优化的诱导条件为:对数生长中期诱导,IPTG浓度为0.3 mmol/L。在优化的培养基和优化的诱导条件下,单位菌体产酶量达745.86 U/g,菌体产酶水平达1 625.97 U/L,为优化前的700 U/L的2.3倍。The nutrient and inducing conditions of cholesterol oxidase by recombinant strain E. coli JM109 were carefully studied in this manuscript and listed as follows： glycerol 10 g/L,tryptone 10 g/L, Yeast power 5 g/L, KH2PO4 2 g/L, K2HPO4 4 g/L, Na2HPO4 · 12H2O 7 g/L, （NH4）2SO4 1.2 g/L,NH4Cl 0. 2 g/L,MgSO4 · 7H2O 1 g/L and 0.3 mmol/L IPTG added to the fermentation broth at the intermediate cell density. Under the optimum conditions, the cholesterol oxidase achieved at 745.86 U/g cell weight, the enzyme activity reached at 1625.97 U/L, is 2.3 times that of pre-optimization.

关 键 词：重组E.coli JM109/pTrc99a—COD 胆固醇氧化酶 培养基优化 诱导条件优化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]