纳米金标记黄曲霉毒素B1新型检测方法  

Nanogold Labeling Based Novel Detection Method for Aflatoxin B_1

作　　者：李井泉[1,2] 毛秀君[1,3] 王周平[1] 王丽[1] 施用晖[1] 

机构地区：[1]食品科学与技术国家重点实验室,江南大学,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122 [3]汉台区北关农业技术综合服务站,陕西汉中723000

出　　处：《食品与生物技术学报》2009年第5期675-681,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(20805019);国家863计划项目(2008AA10Z419);江苏省自然科学基金项目(BK20081603);教育部博士点基金新教师项目(20070295014);江苏省大学生实践创新项目

摘　　要：本文建立了两种基于银增强纳米金标记探针的高灵敏度免疫分析方法。方法一是用黄曲霉毒素B1(AFB1)抗体与金标抗原、待测抗原进行竞争免疫反应,然后加入银增强溶液,以金为核沉积生长银,通过检测光密度来确定待测物中AFB1的含量,该方法的检出限可达到0.01 ng/mL。方法二是在前一种方法的基础上,将银化学溶出,通过化学发光法检测沉积的银量来确定待测物中AFB1的含量,该方法的检出限可达到0.002 ng/mL。The rapid, sensitive measurement of Aflatoxin B1 is of great importance in food salty detection. In the present work, two methods for the competitive heterogeneous immunoassay of aflatoxin B1 （AFB1） was developed. The first one is based on the competitively immune bind to AFB1 antibody between the tested antigen and nanogold--labeled antigen. A silver enhancement process then was conducted to deposit silver around nanogold. Measuring the absorbance of silver at 630 nm can identify the content of AFB1. The detection limit for the proposed method is 0. 01 ng/mL （3σ）. Based upon the first method, the deposited silver was then dissolved to form Ag^＋ , which was measured with a sensitive CL system. And the CL intensity was found to be proportional with the content of AFB1 with a detection limit of 0. 002 ng/mL （3σ）.

关 键 词：纳米金 化学发光 银增强 黄曲霉毒素B1(AFB1) 

分 类 号：TS207.4[轻工技术与工程—食品科学]