维生素C发酵中2-酮-L-古龙酸还原酶的研究  

Study on 2 keto L gulonate Reductase in Vitamin C Fermentation

作　　者：蒋宇扬[1] 郭振勇[1] 靳素英[1] 蔺继尚 张成刚[1] 

机构地区：[1]中科院沈阳应用生态研究所

出　　处：《生物工程学报》1998年第3期339-341,共3页Chinese Journal of Biotechnology

基　　金：国家"八;五"攻关项目

摘　　要：由于２酮Ｌ古龙酸还原酶（简称ＫＧＲ）的存在，理论上造成了Ｖｃ合成前体２酮Ｌ古龙酸（简称２ＫＬＧ）部分被还原成Ｌ艾杜糖酸［１，２］，影响了Ｖｃ二步发酵的产率。而从Ｌ山梨糖到２酮Ｌ古龙酸的转化中，除被ＫＧＲ催化的还原负反应外，均属...keto L gulonate reductase is composed of two Subunits,one with MW 32kDa,the other with 53kDa.Its isoelectric point is pI 3.6.The content of acidic amino acid is 22% of tatal protein.It is found that the rare eanth metal compounds have a surpressive effect of on the activity of 2 keto L gulonate reduetase.Using such rare earith metal compounds in the two steps fermentation of Vc,the fermentation time is shortened by 6 hours and the product of 2 keto L gulonic acid is increased by 10% more.

关 键 词：维生素C发酵 KGR 性质 活性 稀土元素化合物 

分 类 号：Q554[生物学—生物化学] TQ924[轻工技术与工程—发酵工程]